PXD054962

PXD054962 is an original dataset announced via ProteomeXchange.

Title	Novel RNA-Targeted Proteomics Approach Leads to the Identification of a Key Role for YBX1 in HCMV Replication
Description	Understanding the intricate interplay between host factors and viral pathogens remains a focal point of research in molecular virology. Here, we describe a new role for Y-box binding protein 1 (YBX1) during Human Cytomegalovirus (HCMV) infection in regulating the translation of mRNAs encoding the immediate early proteins IE1 and IE2. In this study, we introduce a novel technique for investigating RNA-protein interactions, leveraging the strengths of CRISPR-Cas13, allowing for precise and sensitive exploration of RNA-protein interactions during infection in a temporal manner. Through this approach, we explored the role of YBX1 in HCMV replication, providing a deeper understanding of the molecular host-pathogen interactions. This research not only advances our knowledge of YBX1’s role in viral pathogenesis, but also introduces a powerful tool for unraveling RNA-protein dynamics that can be used in a wide range of biological processes.
HostingRepository	PRIDE
AnnounceDate	2025-02-13
AnnouncementXML	Submission_2025-02-13_11:54:46.583.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Erik Lenarcic
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	biotinylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-16 07:52:17	ID requested
⏵ 1	2025-02-13 11:54:47	announced

10.1073/PNAS.2421155122;

submitter keyword: HCMV, CRISPR-Cas13,human herpesvirus, virology, label-free quantitation, BioID

Nathaniel Moorman
contact affiliation	Department of Microbiology and Immunology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599
contact email	nathaniel_moorman@med.unc.edu
lab head
Erik Lenarcic
contact affiliation	UNC Chapel Hill School of Medicine Department of Microbiology and Immunology
contact email	erik_lenarcic@med.unc.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD054962

PRIDE project URI

• PRIDE
  1. PXD054962
     1. Label: PRIDE project
     2. Name: Novel RNA-Targeted Proteomics Approach Leads to the Identification of a Key Role for YBX1 in HCMV Replication