PXD054933

PXD054933 is an original dataset announced via ProteomeXchange.

Title	Identification of ASC-interacting proteins
Description	To explore the spatiotemporal regulation of ASC speck formation and inflammasome activation, we treated primary WT and Asc–/– bone marrow-derived macrophages (BMDMs) with LPS (500 ng/ml) and ATP (5 mM) to activate NLRP3 inflammasome activation and then performed ASC IP-MS to identify proteins that interacted with ASC. We compared the IP products between WT BMDMs and Asc–/– BMDMs, and found that many proteins specifically interacted with ASC
HostingRepository	PRIDE
AnnounceDate	2026-06-29
AnnouncementXML	Submission_2026-06-28_16:16:56.644.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaopeng Qi
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-15 05:26:20	ID requested
⏵ 1	2026-06-28 16:16:57	announced

10.1038/s41421-026-00898-1;

Mao R, Liu Y, Fan Z, Li Y, Yang L, Xie Q, Dong HP, Bi M, Gao C, Yang Z, Xu T, Qi X, RNA helicase DDX6 governs ASC speck formation in P-bodies and the transition to stress granules via phase separation during inflammasome activation. Cell Discov, 12(1):(2026) [pubmed]

submitter keyword: ASC, mouse BMDM, LPS and ATP, NLRP3 inflammasome

Xiaopeng Qi
contact affiliation	Key Laboratory for Experimental Teratology of the Ministry of Education, Advanced Medical Research Institute, Qilu Hospital, Cheeloo College of Medicine, Shandong University
contact email	xqi@email.sdu.edu.cn
lab head
Xiaopeng Qi
contact affiliation	Key Laboratory for Experimental Teratology of the Ministry of Education, Department of Clinical Laboratory/Qilu Hospital, Advanced Medical Research Institute, Cheeloo College of Medicine, Shandong University
contact email	xqi@email.sdu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/06/PXD054933

PRIDE project URI

• PRIDE
  1. PXD054933
     1. Label: PRIDE project
     2. Name: Identification of ASC-interacting proteins