PXD054919

PXD054919 is an original dataset announced via ProteomeXchange.

Title	Global Profiling of Protein Lactylation, Crotonylation, Succinylation and Phosphorylation in Lung Adenocarcinoma Cell.
Description	The novel protein acylation modifications have played a vital role in protein post-translational modifications. However, the functions and effects of the protein acylation modifications in lung adenocarcinoma are still uncertain. Currently, there is also a lack of global identification of acylation modifications in lung adenocarcinoma cells. Therefore, in this study, we detected 10 currently known acylation modifications in lung adenocarcinoma cells by western blot. Interestingly, we found that the abundance of lysine succinylation (Ksu), crotonylation (Kcr) and lactylation (Kla) is likely higher. Immediately, we identified the above three modifications and phosphorylation by global mass spectrometry-based proteomics in lung adenocarcinoma cells. As a result, we got 3110 Kla sites in 1220 lactylated proteins, 16653 Kcr sites in 4137 crotonylated proteins, 4475 Ksu sites in 1221 succinylated proteins, and 15254 phosphorylation sites in 4139 phosphorylated proteins. In conclusion, our results provide a proteome-wide database to study Kla, Kcr and Ksu and phosphorylation in lung adenocarcinoma, and our bioinformatics results provide new insights into the role of acylation modification in lung adenocarcinoma.
HostingRepository	PRIDE
AnnounceDate	2025-12-01
AnnouncementXML	Submission_2025-11-30_16:10:29.211.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiang He
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: NEWT:9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-15 02:49:17	ID requested
⏵ 1	2025-11-30 16:10:30	announced

10.1038/s41419-025-08113-x;

submitter keyword: Lactylation

Crotonylation

Succinylation

Phosphorylation

Lung Adenocarcinoma

Jiang He
contact affiliation	Radiation Oncology Center, Chongqing University Cancer Hospital, Chongqing 400030, China.
contact email	hejiang1990@hotmail.com
lab head
Jiang He
contact affiliation	Chongqing University Cancer Hospital
contact email	hejiang1990@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD054919

PRIDE project URI

• PRIDE
  1. PXD054919
     1. Label: PRIDE project
     2. Name: Global Profiling of Protein Lactylation, Crotonylation, Succinylation and Phosphorylation in Lung Adenocarcinoma Cell.