PXD054892

PXD054892 is an original dataset announced via ProteomeXchange.

Title	GRASPing experience-dependent protein expression signatures enriched for hippocampal engram cell synapses
Description	Enhanced synaptic wiring onto sparsely distributed memory engram cells supports contextual memory storage and recall, with negative-valence memories exhibiting greater salience. Protein-level adaptations of input-specific synaptic connectivity onto hippocampal CA1 engram cells, however, remains largely unexplored. By combining spatiotemporally restricted synapse labelling, sorting, and mass spectrometry, we generated a discovery-oriented proteomic dataset from samples enriched for CA1 engram cell synapses receiving CA3 input 72 h after neutral context exploration or aversive contextual fear conditioning. Differential analysis relative to an unlabelled comparator identified protein expression signatures associated with synapse structure, efficacy, and strength in CA1 engram cell synapses. Aversive learning induced predominantly postsynaptic protein expression patterns, whereas neutral context skewed towards presynaptic changes. This differential engram cell synapse proteome was enriched for genes linked to cognitive genetic traits and related disorders. Together, these data provide a first hypothesis-generating resource for investigations into the molecular basis of contextual memory at the level of the synapse.
HostingRepository	PRIDE
AnnounceDate	2026-03-25
AnnouncementXML	Submission_2026-03-24_18:55:15.298.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Frank Koopmans
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	methylthiolated residue; monohydroxylated residue
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-14 08:14:58	ID requested
⏵ 1	2026-03-24 18:55:15	announced

Dataset with its publication pending

submitter keyword: None

August B.
contact affiliation	Dept. of Molecular and Cellular Neurobiology, Center for Neurogenomics and Cognitive Research, Amsterdam Neuroscience, Vrije Universiteit Amsterdam, The Netherlands
contact email	guus.smit@vu.nl
lab head
Frank Koopmans
contact affiliation	Vrije Universiteit Amsterdam
contact email	frank.koopmans@vu.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/03/PXD054892

PRIDE project URI

• PRIDE
  1. PXD054892
     1. Label: PRIDE project
     2. Name: GRASPing experience-dependent protein expression signatures enriched for hippocampal engram cell synapses