PXD054883

PXD054883 is an original dataset announced via ProteomeXchange.

Title	Enantioselective OTUD7B fragment discovery through chemoproteomics screening and high-throughput optimisation
Description	Here we present a chemoproteomics fragment screening platform for identifying novel DUB-specific hit matter, that combines activity-based protein profiling with high-throughput chemistry direct-to-biology optimisation to enable rapid elaboration of initial fragment hits against OTU DUBs. Applying these approaches, we identify an enantioselective tool compound for OTUD7B, and validate it using chemoproteomics and biochemical DUB activity assays.
HostingRepository	PRIDE
AnnounceDate	2025-01-14
AnnouncementXML	Submission_2025-01-13_21:54:42.158.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aini Vuorinen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-14 04:57:37	ID requested
⏵ 1	2025-01-13 21:54:42	announced

10.1038/S42004-025-01410-8;

submitter keyword: Chemoproteomics, covalent fragments, data-independent acquisition, activity-based protein profiling, deubiquitinating enzymes

Katrin Rittinger
contact affiliation	Molecular Structure of Cell Signalling Laboratory, The Francis Crick Institute
contact email	katrin.rittinger@crick.ac.uk
lab head
Aini Vuorinen
contact affiliation	The Francis Crick Institute
contact email	aini.vuorinen@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/01/PXD054883

PRIDE project URI

• PRIDE
  1. PXD054883
     1. Label: PRIDE project
     2. Name: Enantioselective OTUD7B fragment discovery through chemoproteomics screening and high-throughput optimisation