PXD054858

PXD054858 is an original dataset announced via ProteomeXchange.

Title	MS-based proteomic characterization of pacmanvirus lostcity virions
Description	The microbial sampling of submarine hydrothermal vent remains challenging, with even fewer studies focused on viruses. Here we report the first isolation of a eukaryotic virus from the Lost City hydrothermal field, by co-culture with the laboratory host Acanthamoeba castellanii. This virus, named pacmanvirus lostcity, is closely related to previously isolated pacmanviruses (strains A23 and S19), clustering in a divergent clade within the long-established family Asfarviridae. Its icosahedral particles are 200 nm in diameter, with an electron-dense core surrounded by an inner membrane. Its genome of 395,708 bp (33% G+C) is predicted to encode 473 proteins. However, besides these standard properties, pacmanvirus lostcity was found associated with a new type of selfish genetic element (pltv), 7 kb in length, whose architecture and gene content are reminiscent of those of transpovirons, hitherto specific to the family Mimiviridae. We performed MS-based proteomic analyses of purified virions to characterize its protein content.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_00:03:10.358.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD054858
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Acanthamoeba castellanii str. Neff; NCBI TaxID: 1257118; scientific name: Asfarviridae; NCBI TaxID: 137992;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-14 02:35:41	ID requested
⏵ 1	2025-05-07 00:03:11	announced

10.6019/PXD054858;

Santini S, Lartigue A, Alempic JM, Cout, é Y, Belmudes L, Brazelton WJ, Lang SQ, Claverie JM, Legendre M, Abergel C, Pacmanvirus isolated from the Lost City hydrothermal field extends the concept of transpoviron beyond the family Mimiviridae. ISME J, 19(1):(2025) [pubmed]

10.1093/ismejo/wraf002;

submitter keyword: purified virions, pacmanviruses,Giant viruses, hydrothermal field, nanoLC-MS/MS

Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054858
     1. Label: PRIDE project
     2. Name: MS-based proteomic characterization of pacmanvirus lostcity virions