PXD054855

PXD054855 is an original dataset announced via ProteomeXchange.

Title	Proteomics in HT1080 WT and GPR56 KO cells
Description	G-protein-coupled receptors (GPCRs) mediate most cellular responses to hormones, neurotransmitters as well as environmental stimulants. However, whether GPCRs participate in modulation of tissue homeostasis through ferroptosis remains unclear. By GPCR cDNA library screening, here we identify that GPR56/ADGRG1 remodels ferroptosis plasticity. Loss of GPR56 sensitizes cells to ferroptosis and deficiency of GPR56 deteriorates ferroptosis-mediated liver injury induced by Doxorubicin (DOX) or ischemia-reperfusion (IR). Mechanistically, GPR56 decreases the abundance of phospholipids containing free polyunsaturated fatty acids (PUFAs) by promoting endocytosis-lysosomal degradation of CD36. By screening a panel of steroid hormones, we identified that 17α-hydroxypregnenolone (17-OH PREG) acts as an agonist of GPR56 to antagonize ferroptosis and efficiently attenuates liver injury before or after insult. Moreover, disease associated GPR56 mutants were unresponsive to 17-OH PREG activation and insufficient to defend against ferroptosis. Together, our findings uncover that 17-OH PREG-GPR56 axis-mediated signal transduction works as a new anti-ferroptotic pathway to maintain liver homeostasis, providing novel insights into the potential therapy for liver injury.
HostingRepository	PRIDE
AnnounceDate	2024-10-11
AnnouncementXML	Submission_2024-10-11_05:08:09.230.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiao Zhuang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-14 02:33:16	ID requested
⏵ 1	2024-10-11 05:08:09	announced

10.1016/J.CMET.2024.09.007;

submitter keyword: HT1080 GPR56 KO； human fibrosarcoma；Proteomics

Bo Chu
contact affiliation	Department of Cell Biology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China.
contact email	201999000129@sdu.edu.cn
lab head
Xiao Zhuang
contact affiliation	Department of Cell Biology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, China.
contact email	zx17865201976@outlook.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD054855

PRIDE project URI

• PRIDE
  1. PXD054855
     1. Label: PRIDE project
     2. Name: Proteomics in HT1080 WT and GPR56 KO cells