PXD054842

PXD054842 is an original dataset announced via ProteomeXchange.

Title	NRAC controls CD36-mediated fatty acid uptake in adipocytes and lipid clearance in vivo.
Description	Adipose tissue is a key pharmacological target to prevent or treat the consequences of obesity. Adipose specific cell surface proteins are especially interesting due to their important roles in orchestrating cellular responses to environmental cues. Nutritionally-regulated adipose and cardiac enriched protein (Nrac) is a small adipocyte specific transmembrane protein with no known function. We show that Nrac modulates CD36 mediated fatty acid uptake, by forming a complex with CD36 and caveolin-1 under low extracellular fatty acid concentrations. Upon loss of Nrac or an increase in extracellular fatty acid levels, leading to reduced Nrac surface localization, CD36 dissociates from caveolin-1 and is internalized via clathrin mediated endocytosis. This results in increased fatty acid uptake into adipocytes, adipocyte hypertrophy, increased fat mass and elevated lipid clearance from the blood in chow diet fed mice. Thus, we unravel a novel regulatory mechanism of adipocyte fatty acid uptake dependent on its extracellular availability
HostingRepository	PRIDE
AnnounceDate	2025-08-04
AnnouncementXML	Submission_2025-08-04_09:43:00.231.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ann-Christine König
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-12 23:55:54	ID requested
⏵ 1	2025-08-04 09:43:00	announced

10.1038/S44318-025-00520-2;

submitter keyword: fatty acid uptake, hypertrophy, clathrin-mediated endocytosis, adipose tissue,CD36

Siegfried Ussar
contact affiliation	Research Unit Adipocytes & Metabolism (ADM), Helmholtz Diabetes Center, Helmholtz Zentrum München, German Research Center for Environmental Health GmbH, 85764, Neuherberg, Germany
contact email	siegfried.ussar@helmholtz-munich.de
lab head
Ann-Christine König
contact affiliation	HMGU Core Facility Proteomics
contact email	ann-christine.koenig@helmholtz-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/08/PXD054842

PRIDE project URI

• PRIDE
  1. PXD054842
     1. Label: PRIDE project
     2. Name: NRAC controls CD36-mediated fatty acid uptake in adipocytes and lipid clearance in vivo.