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PXD054832

PXD054832 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEvidence for substrate control of Cdk phosphorylation timing during the budding yeast cell cycle1757
DescriptionThe main intention of this project is to investigate how Cdk substrate phosphorylation is ordered during the cell cycle. We want to understand what makes a ‘good’ Cdk substrate that is phosphorylated early during the cell cycle, e. g. to promote S phase, as compared to a ‘bad’ Cdk substrate that is phosphorylated much later, e. g. when cells enter mitosis. This could be because a late substrate is indeed a poor Cdk substrate, or it could be that the late substrate is a good substrate for Cdk counteracting phosphatases. The two possibilities are not mutually exclusive. To compare phosphorylation and dephosphorylation dynamics we arrest cells in mitosis when most Cdk substrates are fully phosphorylated. Then, we acutely, and chemically inhibit Cdk. The rate of Cdk substrate dephosphorylation will be measured, which will be an indication of the efficiency of Cdk counteracting phosphatases on each substrate.
HostingRepositoryPRIDE
AnnounceDate2025-04-29
AnnouncementXMLSubmission_2025-04-29_08:06:47.139.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHelen Flynn
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListphosphorylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion Lumos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-12 20:19:05ID requested
12025-04-29 08:06:47announced
Publication List
10.1016/J.CELREP.2025.115534;
Keyword List
submitter keyword: Phosphoproteomics, Cdk inhibition, TMT,Cdk, Cell cycle
Contact List
Frank Uhlmann
contact affiliationChromosome Segregation Laboratory The Francis Crick Institute Midland Road London NW1 1AT
contact emailfrank.uhlmann@crick.ac.uk
lab head
Helen Flynn
contact affiliationThe Francis Crick Institute
contact emailhelen.flynn@crick.ac.uk
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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