PXD054828

PXD054828 is an original dataset announced via ProteomeXchange.

Title	N-Glycosylation profiling in rice
Description	As a ubiquitous and essential posttranslational modification occurred in both plants and animals, protein N-linked glycosylation regulates various important biological processes. Unlike the well studied animal N-glycoproteomes, the landscape of rice N-glycoproteome remains largely unexplored. Here, by developing of a chemical glycoproteomics strategy based on metabolic glycan labeling (MGL) for labeling and enrichment of N-glycoproteins in rice, we report a comprehensive rice N-glycoproteome profiling. In rice seedlings metabolically labeled with N-azidoacetylgalactosamine (GalNAz), followed by conjugation with affinity probes via click chemistry, we identify a total of 426 N-glycosylation sites and 680 N-glycosylated proteins, which are involved in various important biological and pathological processes. In particular, various components of the ER-associated protein degradation (ERAD) machinery are N-glycosylated, which N-glycans play crucial roles for the proper function of ERAD. In addition to providing an invaluable resource for studying the biological function of N-glycosylation in rice, this work demonstrates the versatility of MGL in glycoproteomic proofing for various crop species.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_00:13:04.479.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xilong Li
SpeciesList	scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationList	deamidated residue
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-12 15:08:52	ID requested
⏵ 1	2025-05-07 00:13:05	announced

10.1016/j.mcpro.2024.100883;

Lei C, Li X, Li W, Chen Z, Liu S, Cheng B, Hu Y, Song Q, Qiu Y, Zhou Y, Meng X, Yu H, Zhou W, Chen X, Li J, Chemical Glycoproteomic Profiling in Rice Seedlings Reveals N-glycosylation in the ERAD-L Machinery. Mol Cell Proteomics, 24(2):100883(2025) [pubmed]

submitter keyword: N-Glycosylation,rice

Xilong Li
contact affiliation	Yazhouwan National Laboratory, Sanya 572024, China
contact email	lixilong@yzwlab.cn
lab head
Xilong Li
contact affiliation	Yazhouwan National Laboratory, Sanya, China
contact email	xilongli@genetics.ac.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD054828

PRIDE project URI

• PRIDE
  1. PXD054828
     1. Label: PRIDE project
     2. Name: N-Glycosylation profiling in rice