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PXD054828

PXD054828 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleN-Glycosylation profiling in rice
DescriptionAs a ubiquitous and essential posttranslational modification occurred in both plants and animals, protein N-linked glycosylation regulates various important biological processes. Unlike the well studied animal N-glycoproteomes, the landscape of rice N-glycoproteome remains largely unexplored. Here, by developing of a chemical glycoproteomics strategy based on metabolic glycan labeling (MGL) for labeling and enrichment of N-glycoproteins in rice, we report a comprehensive rice N-glycoproteome profiling. In rice seedlings metabolically labeled with N-azidoacetylgalactosamine (GalNAz), followed by conjugation with affinity probes via click chemistry, we identify a total of 426 N-glycosylation sites and 680 N-glycosylated proteins, which are involved in various important biological and pathological processes. In particular, various components of the ER-associated protein degradation (ERAD) machinery are N-glycosylated, which N-glycans play crucial roles for the proper function of ERAD. In addition to providing an invaluable resource for studying the biological function of N-glycosylation in rice, this work demonstrates the versatility of MGL in glycoproteomic proofing for various crop species.
HostingRepositoryPRIDE
AnnounceDate2025-05-07
AnnouncementXMLSubmission_2025-05-07_00:13:04.479.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterXilong Li
SpeciesList scientific name: Oryza sativa (Rice); NCBI TaxID: 4530;
ModificationListdeamidated residue
InstrumentOrbitrap Fusion Lumos; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-12 15:08:52ID requested
12025-05-07 00:13:05announced
Publication List
10.1016/j.mcpro.2024.100883;
Lei C, Li X, Li W, Chen Z, Liu S, Cheng B, Hu Y, Song Q, Qiu Y, Zhou Y, Meng X, Yu H, Zhou W, Chen X, Li J, Chemical Glycoproteomic Profiling in Rice Seedlings Reveals N-glycosylation in the ERAD-L Machinery. Mol Cell Proteomics, 24(2):100883(2025) [pubmed]
Keyword List
submitter keyword: N-Glycosylation,rice
Contact List
Xilong Li
contact affiliationYazhouwan National Laboratory, Sanya 572024, China
contact emaillixilong@yzwlab.cn
lab head
Xilong Li
contact affiliationYazhouwan National Laboratory, Sanya, China
contact emailxilongli@genetics.ac.cn
dataset submitter
Full Dataset Link List
Dataset FTP location
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