PXD054828 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | N-Glycosylation profiling in rice |
Description | As a ubiquitous and essential posttranslational modification occurred in both plants and animals, protein N-linked glycosylation regulates various important biological processes. Unlike the well studied animal N-glycoproteomes, the landscape of rice N-glycoproteome remains largely unexplored. Here, by developing of a chemical glycoproteomics strategy based on metabolic glycan labeling (MGL) for labeling and enrichment of N-glycoproteins in rice, we report a comprehensive rice N-glycoproteome profiling. In rice seedlings metabolically labeled with N-azidoacetylgalactosamine (GalNAz), followed by conjugation with affinity probes via click chemistry, we identify a total of 426 N-glycosylation sites and 680 N-glycosylated proteins, which are involved in various important biological and pathological processes. In particular, various components of the ER-associated protein degradation (ERAD) machinery are N-glycosylated, which N-glycans play crucial roles for the proper function of ERAD. In addition to providing an invaluable resource for studying the biological function of N-glycosylation in rice, this work demonstrates the versatility of MGL in glycoproteomic proofing for various crop species. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-07_00:13:04.479.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Xilong Li |
SpeciesList | scientific name: Oryza sativa (Rice); NCBI TaxID: 4530; |
ModificationList | deamidated residue |
Instrument | Orbitrap Fusion Lumos; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-08-12 15:08:52 | ID requested | |
⏵ 1 | 2025-05-07 00:13:05 | announced | |
Publication List
10.1016/j.mcpro.2024.100883; |
Lei C, Li X, Li W, Chen Z, Liu S, Cheng B, Hu Y, Song Q, Qiu Y, Zhou Y, Meng X, Yu H, Zhou W, Chen X, Li J, Chemical Glycoproteomic Profiling in Rice Seedlings Reveals N-glycosylation in the ERAD-L Machinery. Mol Cell Proteomics, 24(2):100883(2025) [pubmed] |
Keyword List
submitter keyword: N-Glycosylation,rice |
Contact List
Xilong Li |
contact affiliation | Yazhouwan National Laboratory, Sanya 572024, China |
contact email | lixilong@yzwlab.cn |
lab head | |
Xilong Li |
contact affiliation | Yazhouwan National Laboratory, Sanya, China |
contact email | xilongli@genetics.ac.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD054828
- Label: PRIDE project
- Name: N-Glycosylation profiling in rice