PXD054812

PXD054812 is an original dataset announced via ProteomeXchange.

Title	The proteome at piRNA-target transcripts
Description	Co-transcriptional gene silencing mediated by small RNA pathways is primarily studied in the context of heterochromatin formation. These pathways are known to target nascent RNAs, yet their intersection with cellular processes determining the fate of the nascent transcripts remain unclear. In this study, we investigated the proteome associated with piRNA-target transcripts using biotinylation-based proximity labeling and co-immunoprecipitation assays, followed by quantitative mass spectrometry. Our findings reveal two paralogous proteins, CG31510 and CG7065, as critical piRNA pathway factors that physically connect the nulear RNA exosome, the central cellular 3’ to 5’ exo-ribonuclease complex, to the Piwi-silencing complex through a novel poly-proline binding domain. Notably, we show that the nuclear RNA exosome is required for transposon repression through the Piwi-piRNA pathway in Drosophila. Furthermore, we demonstrate that the three adaptor complexes of the nuclear exosome –PAXT, NEXT, and TRAMP– are redundantly required for degrading piRNA target transcripts. Our findings uncover an evolutionarily conserved principle whereby the nuclear RNA exosome is integral to diverse co-transcriptional silencing processes controlled by small RNAs.
HostingRepository	PRIDE
AnnounceDate	2026-02-27
AnnouncementXML	Submission_2026-02-26_22:38:29.709.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Richard Imre
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: NEWT:7227;
ModificationList	methylthiolated residue; phosphorylated residue; monohydroxylated residue; deamidated residue
Instrument	Orbitrap Eclipse; Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-12 05:36:04	ID requested
⏵ 1	2026-02-26 22:38:30	announced

10.64898/2025.12.16.694471;

submitter keyword: Piwi-piRNA pathway, CG7065, NEXT, PAXT, CG31510, TRAMP, Drosophila, nuclear RNA exosome

Julius Fabian Brennecke
contact affiliation	Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna BioCenter (VBC), 1030 Vienna, Austria.
contact email	julius.brennecke@imba.oeaw.ac.at
lab head
Richard Imre
contact affiliation	IMBA Vienna
contact email	richard.imre@imba.oeaw.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/02/PXD054812

PRIDE project URI

• PRIDE
  1. PXD054812
     1. Label: PRIDE project
     2. Name: The proteome at piRNA-target transcripts