PXD054811

PXD054811 is an original dataset announced via ProteomeXchange.

Title	FADD is recruited to activated STING oligomers initiating caspase mediated NF-κB activation in Drosophila melanogaster
Description	STING is an evolutionarily conserved key regulator of innate immunity. In mammals, STING activates two distinct signaling pathways, the IRF3 and the NF-κB pathways, which act in conjunction to drive antiviral immunity. While the molecular mechanism of STING-mediated IRF3 activation has been untangled by extensive work during the last decade, how STING activates NF-κB signaling remains poorly understood. The versatile FADD/Caspase 8 axis is widely used in various immune signaling pathways throughout the animal kingdom and here we show that this axis participates downstream of STING in the activation of the Drosophila NF-kB like transcription factor Relish. We provide a detailed structural model illustrating how FADD interacts with two separate STING dimers in the activated oligomerized form of STING, thus providing a molecular explanation for the signal-dependent recruitment of FADD. In the classic IMD signaling pathway, FADD interacts with IMD in a structural distinct but functional related manner, highlighting how the two pathways differentially utilize the adapter FADD.
HostingRepository	PRIDE
AnnounceDate	2026-04-09
AnnouncementXML	Submission_2026-04-09_07:41:29.728.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Katarzyna Kjøge
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: NEWT:7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Eclipse

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-12 05:24:19	ID requested
⏵ 1	2026-04-09 07:41:30	announced

10.1038/S44318-026-00761-9;

submitter keyword: co-IP MS, STING singaling, FADD,Innate immunity, NF-κB, Drosophila melanogaster

Rune Hartmann
contact affiliation	Department of Molecular Biology and Genetics, Aarhus University
contact email	rh@mbg.au.dk
lab head
Katarzyna Kjøge
contact affiliation	Aarhus University
contact email	kkjoege@mbg.au.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD054811

PRIDE project URI

• PRIDE
  1. PXD054811
     1. Label: PRIDE project
     2. Name: FADD is recruited to activated STING oligomers initiating caspase mediated NF-κB activation in Drosophila melanogaster