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PXD054810

PXD054810 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphoproteomic response underpinning cardiac organoid maturation by electronic pacing and directed maturation media
DescriptionCardiac maturation is an important developmental phase where there are profound biological and functional changes after birth in mammals. Herein, we use our profiling of human heart maturation in vivo to identify key drivers of maturation in our human cardiac organoid (hCO) model. After screening of various metabolism modulating factors, we established a directed maturation protocol to induce mature cardiac expression. We next compared directed maturation treatment to electrical pacing using phosphoproteomics in order to assess the similarities in the induction of maturation. The electrical pacing protocol utilized a custom platform, where we added Heart-Dyno inserts into C-pace system in 24-well plates enabling 120 bpm pacing for 5 minutes without causing toxicity. In this dataset, we compared 3 replicates of CTRL (our original serum free organoid protocol), electrical pacing (the standard protocol for maturation of cardiac stem cells), and directed maturation protocol (DM, new protocol) through phosphoproteomics.
HostingRepositoryPRIDE
AnnounceDate2025-04-17
AnnouncementXMLSubmission_2025-04-16_16:53:21.285.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterHarley Robinson
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue; deamidated residue; iodoacetamide derivatized residue
InstrumentQ Exactive HF; Q Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-12 05:19:53ID requested
12025-04-16 16:53:21announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: cardiac organoids, maturation, phosphorylation, pacing
Contact List
Prof James Hudson
contact affiliationCardiac Bioengineering group, QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia
contact emailjames.hudson@qimrberghofer.edu.au
lab head
Harley Robinson
contact affiliationQIMR Berghofer Medical Research Institute
contact emailHarley.robinson@uqconnect.edu.au
dataset submitter
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Dataset FTP location
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