PXD054810

PXD054810 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic response underpinning cardiac organoid maturation by electronic pacing and directed maturation media
Description	Cardiac maturation is an important developmental phase where there are profound biological and functional changes after birth in mammals. Herein, we use our profiling of human heart maturation in vivo to identify key drivers of maturation in our human cardiac organoid (hCO) model. After screening of various metabolism modulating factors, we established a directed maturation protocol to induce mature cardiac expression. We next compared directed maturation treatment to electrical pacing using phosphoproteomics in order to assess the similarities in the induction of maturation. The electrical pacing protocol utilized a custom platform, where we added Heart-Dyno inserts into C-pace system in 24-well plates enabling 120 bpm pacing for 5 minutes without causing toxicity. In this dataset, we compared 3 replicates of CTRL (our original serum free organoid protocol), electrical pacing (the standard protocol for maturation of cardiac stem cells), and directed maturation protocol (DM, new protocol) through phosphoproteomics.
HostingRepository	PRIDE
AnnounceDate	2025-04-17
AnnouncementXML	Submission_2025-04-16_16:53:21.285.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harley Robinson
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-12 05:19:53	ID requested
⏵ 1	2025-04-16 16:53:21	announced

Dataset with its publication pending

submitter keyword: cardiac organoids, maturation, phosphorylation, pacing

Prof James Hudson
contact affiliation	Cardiac Bioengineering group, QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia
contact email	james.hudson@qimrberghofer.edu.au
lab head
Harley Robinson
contact affiliation	QIMR Berghofer Medical Research Institute
contact email	Harley.robinson@uqconnect.edu.au
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD054810

PRIDE project URI

• PRIDE
  1. PXD054810
     1. Label: PRIDE project
     2. Name: Phosphoproteomic response underpinning cardiac organoid maturation by electronic pacing and directed maturation media