PXD054810 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteomic response underpinning cardiac organoid maturation by electronic pacing and directed maturation media |
Description | Cardiac maturation is an important developmental phase where there are profound biological and functional changes after birth in mammals. Herein, we use our profiling of human heart maturation in vivo to identify key drivers of maturation in our human cardiac organoid (hCO) model. After screening of various metabolism modulating factors, we established a directed maturation protocol to induce mature cardiac expression. We next compared directed maturation treatment to electrical pacing using phosphoproteomics in order to assess the similarities in the induction of maturation. The electrical pacing protocol utilized a custom platform, where we added Heart-Dyno inserts into C-pace system in 24-well plates enabling 120 bpm pacing for 5 minutes without causing toxicity. In this dataset, we compared 3 replicates of CTRL (our original serum free organoid protocol), electrical pacing (the standard protocol for maturation of cardiac stem cells), and directed maturation protocol (DM, new protocol) through phosphoproteomics. |
HostingRepository | PRIDE |
AnnounceDate | 2025-04-17 |
AnnouncementXML | Submission_2025-04-16_16:53:21.285.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Harley Robinson |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | phosphorylated residue; deamidated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive HF; Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-08-12 05:19:53 | ID requested | |
⏵ 1 | 2025-04-16 16:53:21 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: cardiac organoids, maturation, phosphorylation, pacing |
Contact List
Prof James Hudson |
contact affiliation | Cardiac Bioengineering group, QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia |
contact email | james.hudson@qimrberghofer.edu.au |
lab head | |
Harley Robinson |
contact affiliation | QIMR Berghofer Medical Research Institute |
contact email | Harley.robinson@uqconnect.edu.au |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD054810
- Label: PRIDE project
- Name: Phosphoproteomic response underpinning cardiac organoid maturation by electronic pacing and directed maturation media