PXD054803

PXD054803 is an original dataset announced via ProteomeXchange.

Title	MS-based identification of proteins localized in viral factory of mimivirus
Description	Mimivirus is a well-studied member of the family Mimiviridae which are ubiquitous in the environment. It is a fully cytoplasmic GV which is internalized into the host cell by phagocytosis. The acidic compartment of the phagosome, likely in conjugation with other unknown signals, triggers the opening of the stargate structure located at an apex of the icosahedral capsid. Once opened, the fusion of the membrane of the phagosome with the virion's internal membrane leads to the release in the cytoplasm of the core. The core contains the genome of the virus and the machinery needed to establish infection. The core consequently develops to establish a viral factory (VF), a highly dynamic and complex organelle-like structure. VF not only protects the viral DNA from cellular insults but also segregates DNA replication and transcription from translation. To identify client proteins of the viral factory, we performed co-immunoprecipitation experiments using resident protein of this strcture as baits.
HostingRepository	PRIDE
AnnounceDate	2025-08-21
AnnouncementXML	Submission_2025-08-21_04:49:24.959.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD054803
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yohann Couté
SpeciesList	scientific name: Acanthamoeba castellanii mimivirus; NCBI TaxID: 1899318; scientific name: Acanthamoeba castellanii; NCBI TaxID: 5755;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-12 02:30:31	ID requested
⏵ 1	2025-08-21 04:49:25	announced

10.6019/PXD054803;

submitter keyword: Mimivirus, viral factory, co-immunoprecipitation, nanoLC-MS/MS

Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
lab head
Yohann Couté
contact affiliation	EDyP
contact email	yohann.coute@cea.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054803
     1. Label: PRIDE project
     2. Name: MS-based identification of proteins localized in viral factory of mimivirus