PXD054802

PXD054802 is an original dataset announced via ProteomeXchange.

Title	PP2A Phosphatase Regulatory Subunit PPP2R3C is a New Positive Regulator of the Hedgehog Signaling Pathway
Description	Cellular signaling pathways rely on posttranslational modifications (PTMs) to finely regulate protein functions, particularly transcription factors. The Hedgehog (Hh) signaling cascade, crucial for embryonic development and tissue homeostasis, is susceptible to aberrations that lead to developmental anomalies and various cancers. At the core of Hh signaling are Gli proteins, whose dynamic balance between activator (GliA) and repressor (GliR) states shapes cellular outcomes. Phosphorylation, orchestrated by multiple kinases, is pivotal in regulating Gli activity. While kinases in this context have been extensively studied, the role of protein phosphatases, particularly Protein Phosphatase 2A (PP2A), remains less explored. This study unveils a novel role for the B'' gamma subunit of PP2A, PPP2R3C, in Hh signaling regulation. PPP2R3C interacts with Gli proteins, and its disruption reduces Hedgehog pathway activity. Moreover, we establish a connection between PPP2R3C and MEKK1 kinase in Gli protein phosphorylation, underscoring the intricate interplay between kinases and phosphatases in Hh signaling pathway. This study sheds light on the previously understudied role of protein phosphatases in Hh signaling and provides insights into their significance in cellular regulation.
HostingRepository	PRIDE
AnnounceDate	2025-06-10
AnnouncementXML	Submission_2025-06-10_04:48:07.339.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rita Derua
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	ZenoTOF 7600

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-12 02:25:10	ID requested
⏵ 1	2025-06-10 04:48:08	announced

Baran B, Derua R, Janssens V, Niewiadomski P, PP2A phosphatase regulatory subunit PPP2R3C is a new positive regulator of the hedgehog signaling pathway. Cell Signal, 123():111352(2024) [pubmed]

10.1016/j.cellsig.2024.111352;

submitter keyword: PPP2R3C

PP2A

phosphatases

Hedgehog signaling

Gli

phosphorylation

Dr. Pawel Niewiadomski
contact affiliation	Centre of New Technologies, University of Warsaw, 02-097, Warsaw, Poland
contact email	p.niewiadomski@cent.uw.edu.pl
lab head
Rita Derua
contact affiliation	KU Leuven
contact email	rita.derua@kuleuven.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054802
     1. Label: PRIDE project
     2. Name: PP2A Phosphatase Regulatory Subunit PPP2R3C is a New Positive Regulator of the Hedgehog Signaling Pathway