PXD054796

PXD054796 is an original dataset announced via ProteomeXchange.

Title	Spatiotemporally-resolved mapping of extracellular proteomes via in vivo-compatible TyroID
Description	Extracellular proteins play pivotal roles in both intracellular signaling and intercellular communications in health and disease. While recent advancements in proximity labeling (PL) methods, such as peroxidase- and photocatalyst-based approaches, have facilitated the resolution of extracellular proteomes, their in vivo compatibility remains limited. Here, we report TyroID, an in vivo-compatible PL method for unbiased mapping of extracellular proteins with high spatiotemporal resolution. TyroID employs plant- and bacteria-derived tyrosinases to produce reactive o-quinone intermediates, enabling the labeling of multiple residues on endogenous proteins with bioorthogonal handles, thereby allowing for their identification via chemical proteomics. We validate TyroID's specificity by mapping extracellular proteomes and HER2-neighboring proteins using nanobody-directed recombinant tyrosinases. Demonstrating its superiority over other PL methods, TyroID enables in vivo mapping of extracellular proteomes, including mapping HER2-proximal proteins in tumor xenografts, quantifying the turnover of plasma proteins and labeling hippocampal-specific proteomes in live mouse brains. TyroID emerges as a potent tool for investigating protein localization and molecular interactions within living organisms.
HostingRepository	iProX
AnnounceDate	2024-11-21
AnnouncementXML	Submission_2025-01-21_18:23:48.307.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yankun Wang
SpeciesList	scientific name: Bos taurus; NCBI TaxID: 9913; scientific name: Homo sapiens; NCBI TaxID: 9606; scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-11 20:22:07	ID requested
⏵ 1	2025-01-21 18:23:48	announced

Dataset with its publication pending

submitter keyword: proximity labeling

Wei Qin
contact affiliation	Tsinghua University
contact email	weiqin@tsinghua.edu.cn
lab head
Yankun Wang
contact affiliation	Tsinghua University
contact email	yankun_wang@hotmail.com
dataset submitter

iProX dataset URI