PXD054784

PXD054784 is an original dataset announced via ProteomeXchange.

Title	Identification of proteins from SECphi18 lacking Ksap1 recognized by the KpAvs2 bacterial defense system during infection of Escherichia coli.
Description	Antiviral STANDs (Avs) are bacterial anti-phage proteins that are considered as the evolutionary ancestors of immune pattern-recognition receptors of the NLR family. Following the recognition of a conserved phage protein, Avs proteins exhibit cellular toxicity and abort phage propagation by killing the infected cell. Type 2 Avs proteins (Avs2) were suggested to recognize the large terminase subunit of the phage by direct binding as a signature of phage infection based on co-expression assays. Here, we analyzed the binding partners of a type 2 Avs protein from Klebsiella pneumoniae (KpAvs2) expressed in Escherichia coli. Previous analysis revealed that, during phage SECphi18 infection, KpAvs2 recognized a small protein of unknown function, Ksap1. We repeated the experiment with a SECphi18 phage mutated for Ksap1 to see if KpAvs2 could recognize other proteins, such as the terminase, in absence of Ksap1.
HostingRepository	PRIDE
AnnounceDate	2024-12-04
AnnouncementXML	Submission_2024-12-04_07:01:06.633.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nathalie Bechon
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-11 07:45:53	ID requested
⏵ 1	2024-12-04 07:01:07	announced

10.1038/S41467-024-54214-0;

submitter keyword: defense system, pattern recognition,bacteriophage

Rotem Sorek
contact affiliation	Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
contact email	rotem.sorek@weizmann.ac.il
lab head
Nathalie Bechon
contact affiliation	Weizmann Institute of Science
contact email	nathalie.bechon@weizmann.ac.il
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/12/PXD054784

PRIDE project URI

• PRIDE
  1. PXD054784
     1. Label: PRIDE project
     2. Name: Identification of proteins from SECphi18 lacking Ksap1 recognized by the KpAvs2 bacterial defense system during infection of Escherichia coli.