PXD054773

PXD054773 is an original dataset announced via ProteomeXchange.

Title	Data-independent acquisition proteomics enhances cellular nucleic acid binding protein characterization in colitis data1
Description	We developed a robust workflow for in-depth analysis of NABPs, which combining immobilized metal affinity chromatography capture and data-independent acquisition. This workflow enhances the identification and quantification of NABPs. We then applied this workflow to colitis mouse and discovered epigenetic processes, particularly histone methylation was significantly changed in spleen.
HostingRepository	PRIDE
AnnounceDate	2025-12-01
AnnouncementXML	Submission_2025-11-30_16:09:44.845.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Huiyu Wang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-10 02:52:20	ID requested
⏵ 1	2025-11-30 16:09:45	announced

10.1093/nar/gkaf1167;

Wang H, Zhu J, Li S, Zhang L, Guo M, Zhao J, Zhang Y, Cao C, Hu S, Xiao H, Massive deciphering of the tissue nucleic acid-binding proteome via affinity chromatography integrated with data-independent acquisition-based proteomics. Nucleic Acids Res, 53(21):(2025) [pubmed]

submitter keyword: nucleic acid binding protemoics, LC/MS-MS,Colitis

Hua Xiao
contact affiliation	State Key Laboratory of Microbial Metabolism, Joint International Research Laboratory of Metabolic & Developmental Sciences, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, China.
contact email	huaxiao@sjtu.edu.cn
lab head
Huiyu Wang
contact affiliation	18272583259
contact email	lizlemon@sjtu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/12/PXD054773

PRIDE project URI

• PRIDE
  1. PXD054773
     1. Label: PRIDE project
     2. Name: Data-independent acquisition proteomics enhances cellular nucleic acid binding protein characterization in colitis data1