PXD054767

PXD054767 is an original dataset announced via ProteomeXchange.

Title	Analysis of Protein Carrier AAV by LC-MS/MS
Description	AAV is widely used for efficient delivery of DNA payloads. The extent to which the AAV capsid can be used to deliver a protein payload is unexplored. Here, we report engineered AAV capsids that directly package proteins – Protein Carrier AAV (pcAAV). Nanobodies inserted into the interior of the capsid mediate packaging of a cognate protein, including Green Fluorescent Protein (GFP), Streptococcus pyogenes Cas9, Cre recombinase, and the engineered peroxidase APEX2. We show that protein packaging efficiency is affected by the nanobody insertion position, the capsid protein isoform into which the nanobody is inserted, and the subcellular localization of the packaged protein during recombinant AAV capsid production; each of these factors can be rationally engineered to optimize protein packaging efficiency. We demonstrate that protein packaged within pcAAV retain their enzymatic activity and that pcAAV can bind and enter the cell to deliver the protein payload. Establishing pcAAV as a protein delivery platform expands the utility of AAV as a therapeutic and research tool.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-07_00:10:05.206.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD054767
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ryan Sorensen
SpeciesList	scientific name: Adeno-associated virus 2 Srivastava/1982; NCBI TaxID: 648242;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-09 14:46:27	ID requested
⏵ 1	2025-05-07 00:10:06	announced

10.6019/PXD054767;

10.1021/acsnano.5c01498;

Hoffmann MD, Sorensen RJ, Extross A, He Y, Schmidt D, Protein Carrier Adeno-Associated Virus. ACS Nano, 19(12):12308-12322(2025) [pubmed]

submitter keyword: LC-MSMS,Adeno-associated virus, DIA, MaxQuant

Daniel Schmidt
contact affiliation	Department of Genetics, Cell Biology & Development, University of Minnesota
contact email	schmida@umn.edu
lab head
Ryan Sorensen
contact affiliation	University of Minnesota
contact email	soren940@umn.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD054767

PRIDE project URI

• PRIDE
  1. PXD054767
     1. Label: PRIDE project
     2. Name: Analysis of Protein Carrier AAV by LC-MS/MS