PXD054767
PXD054767 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Analysis of Protein Carrier AAV by LC-MS/MS |
Description | AAV is widely used for efficient delivery of DNA payloads. The extent to which the AAV capsid can be used to deliver a protein payload is unexplored. Here, we report engineered AAV capsids that directly package proteins – Protein Carrier AAV (pcAAV). Nanobodies inserted into the interior of the capsid mediate packaging of a cognate protein, including Green Fluorescent Protein (GFP), Streptococcus pyogenes Cas9, Cre recombinase, and the engineered peroxidase APEX2. We show that protein packaging efficiency is affected by the nanobody insertion position, the capsid protein isoform into which the nanobody is inserted, and the subcellular localization of the packaged protein during recombinant AAV capsid production; each of these factors can be rationally engineered to optimize protein packaging efficiency. We demonstrate that protein packaged within pcAAV retain their enzymatic activity and that pcAAV can bind and enter the cell to deliver the protein payload. Establishing pcAAV as a protein delivery platform expands the utility of AAV as a therapeutic and research tool. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-07 |
AnnouncementXML | Submission_2025-05-07_00:10:05.206.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD054767 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Ryan Sorensen |
SpeciesList | scientific name: Adeno-associated virus 2 Srivastava/1982; NCBI TaxID: 648242; |
ModificationList | acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2024-08-09 14:46:27 | ID requested | |
⏵ 1 | 2025-05-07 00:10:06 | announced |
Publication List
10.6019/PXD054767; |
10.1021/acsnano.5c01498; |
Hoffmann MD, Sorensen RJ, Extross A, He Y, Schmidt D, Protein Carrier Adeno-Associated Virus. ACS Nano, 19(12):12308-12322(2025) [pubmed] |
Keyword List
submitter keyword: LC-MSMS,Adeno-associated virus, DIA, MaxQuant |
Contact List
Daniel Schmidt | |
---|---|
contact affiliation | Department of Genetics, Cell Biology & Development, University of Minnesota |
contact email | schmida@umn.edu |
lab head | |
Ryan Sorensen | |
contact affiliation | University of Minnesota |
contact email | soren940@umn.edu |
dataset submitter |
Full Dataset Link List
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD054767 |
PRIDE project URI |
Repository Record List
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