PXD054765 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterization of endosomes and interactions from TMEM230 and TMEM9/9B mutants in human neurons |
Description | EndoMAP aims to systematically dissect the human endosomal protein complexes. To examine TMEM9 and TMEM230 interactions, we generated TMEM230 and TMEM9 knockouts (KOs) using CRISPR/Cas9 in human embryonic stem (ES) cells and converted the cells to induced neurons (iNeurons). TMEM230 and TMEM9 were immunoprecipitated (IP) on day-21 iNeurons and analyzed by TMT-MS, using the respective KOs as a negative control. In addition, TMEM230 immunoprecipitations were performed in TMEM230 KO iNeurons expressing WT and individual disease variant of TMEM230 (Y29C, R78L, and X121W), as well as a triple mutant carrying all three variants. To examine the impact of TMEM230 mutations and TMEM9/9B loss on total and endosomal proteome, we additionally generated TMEM230(X121W) and TMEM9/TMEM9B double knockout (DKO) in ES cells. The proteome of post-nuclear supernatant (PNS) and purified endosomes (Endo-IP) from WT, TMEM230 KO, and TMEM230(X121W) iNeurons were analyzed by TMT-MS. The proteome of PNS and purified endosomes (Endo-IP) from TMEM9 KO and two clones of TMEM9/9B DKO iNeurons were analyzed by TMT-MS. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-30 |
AnnouncementXML | Submission_2025-05-30_02:37:41.575.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD054765 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Miguel A. Gonzalez-Lozano |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Eclipse |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-08-09 12:23:50 | ID requested | |
1 | 2025-03-29 12:25:20 | announced | |
⏵ 2 | 2025-05-30 02:37:41 | announced | 2025-05-30: Updated project metadata. |
Publication List
Keyword List
submitter keyword: TMT, Human, TMEM9, Endosomes, iNeurons, TMEM230,EndoMAP |
Contact List
J. Wade Harper |
contact affiliation | Department of Cell Biology, Harvard Medical School, Boston MA |
contact email | wade_harper@hms.harvard.edu |
lab head | |
Miguel A. Gonzalez-Lozano |
contact affiliation | Department of Cell Biology, Harvard Medical School, Boston MA |
contact email | miguel_gonzalezlozano@hms.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD054765
- Label: PRIDE project
- Name: Characterization of endosomes and interactions from TMEM230 and TMEM9/9B mutants in human neurons