PXD054755

PXD054755 is an original dataset announced via ProteomeXchange.

Title	Enhancing phage therapy by coating single bacteriophage-infected bacteria with polymer to preserve phage vitality
Description	The efficacy of bacteriophages in treating bacterial infections largely depends on the phages’ vitality, which is impaired when they are naturally released from their hosts, as well as by culture media, manufacturing processes and other insults. Here, by wrapping phage-invaded bacteria individually with a polymeric nanoscale coating to preserve the microenvironment on phage-induced bacterial lysis, we show that, compared with naturally released phages, which have severely degraded proteins in their tail, the vitality of phages isolated from polymer-coated bacteria is maintained. Such latent phages could also be better amplified, and they more efficiently bound and lysed bacteria when clearing bacterial biofilms. In mice with bacterially induced enteritis and associated arthritis, latent phages released from orally administered bacteria coated with a polymer that dissolves at neutral pH had higher bioavailability and led to substantially better therapeutic outcomes than the administration of uncoated phages.
HostingRepository	PRIDE
AnnounceDate	2025-02-05
AnnouncementXML	Submission_2025-02-04_18:33:23.966.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guocheng Xie
SpeciesList	scientific name: Salmonella phage SFP10; NCBI TaxID: 1080800;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-09 05:40:28	ID requested
⏵ 1	2025-02-04 18:33:24	announced

10.1038/S41551-025-01354-3;

submitter keyword: bacteriophage single-cell-nanocoating phage therapy

Jinyao liu
contact affiliation	Shanghai Jiao Tong University
contact email	jyliu@sjtu.edu.cn
lab head
Guocheng Xie
contact affiliation	Shanghai Jiao Tong University
contact email	xiegc@sjtu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD054755

PRIDE project URI

• PRIDE
  1. PXD054755
     1. Label: PRIDE project
     2. Name: Enhancing phage therapy by coating single bacteriophage-infected bacteria with polymer to preserve phage vitality