PXD054755 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Enhancing phage therapy by coating single bacteriophage-infected bacteria with polymer to preserve phage vitality |
Description | The efficacy of bacteriophages in treating bacterial infections largely depends on the phages’ vitality, which is impaired when they are naturally released from their hosts, as well as by culture media, manufacturing processes and other insults. Here, by wrapping phage-invaded bacteria individually with a polymeric nanoscale coating to preserve the microenvironment on phage-induced bacterial lysis, we show that, compared with naturally released phages, which have severely degraded proteins in their tail, the vitality of phages isolated from polymer-coated bacteria is maintained. Such latent phages could also be better amplified, and they more efficiently bound and lysed bacteria when clearing bacterial biofilms. In mice with bacterially induced enteritis and associated arthritis, latent phages released from orally administered bacteria coated with a polymer that dissolves at neutral pH had higher bioavailability and led to substantially better therapeutic outcomes than the administration of uncoated phages. |
HostingRepository | PRIDE |
AnnounceDate | 2025-02-05 |
AnnouncementXML | Submission_2025-02-04_18:33:23.966.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Guocheng Xie |
SpeciesList | scientific name: Salmonella phage SFP10; NCBI TaxID: 1080800; |
ModificationList | TMT6plex-126 reporter+balance reagent acylated residue |
Instrument | LTQ Orbitrap Elite |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-08-09 05:40:28 | ID requested | |
⏵ 1 | 2025-02-04 18:33:24 | announced | |
Publication List
Keyword List
submitter keyword: bacteriophage single-cell-nanocoating phage therapy |
Contact List
Jinyao liu |
contact affiliation | Shanghai Jiao Tong University |
contact email | jyliu@sjtu.edu.cn |
lab head | |
Guocheng Xie |
contact affiliation | Shanghai Jiao Tong University |
contact email | xiegc@sjtu.edu.cn |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD054755 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD054755
- Label: PRIDE project
- Name: Enhancing phage therapy by coating single bacteriophage-infected bacteria with polymer to preserve phage vitality