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PXD054755

PXD054755 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleEnhancing phage therapy by coating single bacteriophage-infected bacteria with polymer to preserve phage vitality
DescriptionThe efficacy of bacteriophages in treating bacterial infections largely depends on the phages’ vitality, which is impaired when they are naturally released from their hosts, as well as by culture media, manufacturing processes and other insults. Here, by wrapping phage-invaded bacteria individually with a polymeric nanoscale coating to preserve the microenvironment on phage-induced bacterial lysis, we show that, compared with naturally released phages, which have severely degraded proteins in their tail, the vitality of phages isolated from polymer-coated bacteria is maintained. Such latent phages could also be better amplified, and they more efficiently bound and lysed bacteria when clearing bacterial biofilms. In mice with bacterially induced enteritis and associated arthritis, latent phages released from orally administered bacteria coated with a polymer that dissolves at neutral pH had higher bioavailability and led to substantially better therapeutic outcomes than the administration of uncoated phages.
HostingRepositoryPRIDE
AnnounceDate2025-02-05
AnnouncementXMLSubmission_2025-02-04_18:33:23.966.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterGuocheng Xie
SpeciesList scientific name: Salmonella phage SFP10; NCBI TaxID: 1080800;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue
InstrumentLTQ Orbitrap Elite
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-09 05:40:28ID requested
12025-02-04 18:33:24announced
Publication List
10.1038/S41551-025-01354-3;
Keyword List
submitter keyword: bacteriophage single-cell-nanocoating phage therapy
Contact List
Jinyao liu
contact affiliationShanghai Jiao Tong University
contact emailjyliu@sjtu.edu.cn
lab head
Guocheng Xie
contact affiliationShanghai Jiao Tong University
contact emailxiegc@sjtu.edu.cn
dataset submitter
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Dataset FTP location
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PRIDE project URI
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