PXD054736

PXD054736 is an original dataset announced via ProteomeXchange.

Title	Venom proteomics of Leptopilina syphax and Leptopilina drosophilae
Description	Venom protein fluid was solubilized in SDT lysis buffer (4% SDS, 100mM Tris-HCl, 1mM DTT, pH 7.6). The detergent, DTT and other low-molecular-weight components were removed by repeated ultrafiltration (Microcon units, 30 kD) using UA buffer (8 M Urea, 150 mM Tris-HCl pH 8.0). The protein suspensions were digested with trypsin (Promega) in 100mM NH4HCO3 buffer overnight at 37 °C, and the resulting peptides were collected as a filtrate. Peptide fragmentation using C18 Cartridge After the peptides were lyophilized, 0.1% formic acid solution was added to reconstitute, and the peptides were quantified (OD280). The peptide separation was performed using the Easy nLC HPLC liquid system with nanoliter flow rates. The peptides were separated by chromatography and analyzed by mass spectrometry using a Q-Exactive mass spectrometer (Thermo Fisher Scientific).
HostingRepository	iProX
AnnounceDate	2024-08-08
AnnouncementXML	Submission_2025-04-22_21:18:23.384.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Zhi Dong
SpeciesList	scientific name: Leptopilina; NCBI TaxID: 63429;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-08 21:49:01	ID requested
⏵ 1	2025-04-22 21:18:23	announced

Dataset with its publication pending

submitter keyword: venom, Leptopilina, wasp

Jianhua Huang
contact affiliation	Institute of Insect Sciences, Zhejiang University
contact email	jhhuang@zju.edu.cn
lab head
Zhi Dong
contact affiliation	Institute of Insect Sciences, Zhejiang University
contact email	dongz@zju.edu.cn
dataset submitter

iProX dataset URI