PXD054714 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Human microbiome LC-MS Proteomics in Crohn's disease patients |
| Description | Introduction. A substantial number of Crohn’s disease (CD) patients experience side-effects and/or non-response to medical drugs. In part, this might be attributed to the interaction of the intestinal microbiome with xenobiotics. The aim of this study was to explore the effect of common CD drugs on the patient’s microbiome in vitro. Methods The fecal microbiome of each of 5 CD patients was exposed to 42 μg/ml budesonide, 55 μg/ml 6-mercaptopurine (6-MP), 5 or 15 μg/ml tofacitinib, or DMSO-control in defined culture medium in an anaerobic chamber at 37°C for 24 hours. Subsequently, DNA and proteins were isolated and subjected to 16 rRNA gene amplicon sequencing and LC-MS proteomic analysis, respectively. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-04-27 |
| AnnouncementXML | Submission_2026-04-26_17:07:35.429.xml |
| DigitalObjectIdentifier | https://doi.org/10.6019/PXD054714 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Ronny Mohren |
| SpeciesList | scientific name: gut metagenome; NCBI TaxID: NEWT:749906; scientific name: human gut metagenome; NCBI TaxID: NEWT:408170; |
| ModificationList | acetylated residue; iodoacetamide derivatized residue |
| Instrument | Q Exactive HF |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-08-08 08:08:05 | ID requested | |
| ⏵ 1 | 2026-04-26 17:07:36 | announced | |
Publication List
| 10.1186/s12915-026-02569-9; |
| Becker HEF, Mohren R, Le NG, Derijks LJJ, Jonkers DMAE, Penders J, 6-mercaptopurine and tofacitinib alter microbial protein expression but not composition in fecal microbiota incubations from Crohn's disease patients. BMC Biol, 24(1):(2026) [pubmed] |
| 10.6019/PXD054714; |
Keyword List
| submitter keyword: Human, Crohn's disease, drugs, Microbiome, LC-MS, Proteomics |
Contact List
| Ron Heeren / Ronny Mohren |
|---|
| contact affiliation | Maastricht University, FHML, M4i |
| contact email | r.mohren@maastrichtuniversity.nl |
| lab head | |
| Ronny Mohren |
|---|
| contact affiliation | Maastricht University |
| contact email | r.mohren@maastrichtuniversity.nl |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/04/PXD054714 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD054714
- Label: PRIDE project
- Name: Human microbiome LC-MS Proteomics in Crohn's disease patients
