PXD054708

PXD054708 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomics analysis of TAMs with NEK2 overexpression
Description	NEK2 is considered a key factor in the maintenance of development and function of B cells in multiple myeloma (MM). Importantly, we previously demonstrated that PD-L1 is a substrate of NEK2, and combinatorial inhibition of NEK2 and PD-L1 largely improves the therapeutic efficacy of pancreatic cancer in pre-clinical models. But the expression and function of NEK2 in HCC-related TAMs have not yet been studied. This analysis revealed changes in the proteome due to the overexpression of NEK2 in TAMs, providing a valuable reference for further functional investigations.
HostingRepository	PRIDE
AnnounceDate	2025-05-26
AnnouncementXML	Submission_2025-05-25_16:09:14.224.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaozhen Zhang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-08 05:46:34	ID requested
⏵ 1	2025-05-25 16:09:15	announced

Zhang X, Lao M, Sun K, Yang H, He L, Liu X, Liu L, Zhang S, Guo C, Wang S, Shi J, Zhang X, Xu D, Lu X, Bai X, Liang T, Sphingolipid synthesis in tumor-associated macrophages confers immunotherapy resistance in hepatocellular carcinoma. Sci Adv, 11(21):eadv0558(2025) [pubmed]

10.1126/sciadv.adv0558;

submitter keyword: NEK2

Phosphoproteomics;TAMs

pancreatic cancer

Xiaozhen Zhang
contact affiliation	First Affiliated Hospital, School of Medicine, Zhejiang University
contact email	zhangxiaozhen@zju.edu.cn
lab head
Xiaozhen Zhang
contact affiliation	Department of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital, School of Medicine, Zhejiang University
contact email	zhangxiaozhen@zju.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD054708

PRIDE project URI

• PRIDE
  1. PXD054708
     1. Label: PRIDE project
     2. Name: Phosphoproteomics analysis of TAMs with NEK2 overexpression