PXD054704

PXD054704 is an original dataset announced via ProteomeXchange.

Title	A mitochondrial sirtuin shapes the intestinal microbiota by controlling lysozyme expression
Description	Sirtuins are sensors that orchestrate cellular responses to adapt to changing situations. Here, we show that a protein-restricted diet induces strongly induces increased intestinal expression of the sole Drosophila mitochondrial sirtuin, dSirt4. To elucidate the effects of deregulated dSirt4 expression in the intestine, we analyzed dSirt4-deficient animals. These dSirt4-deficient flies show substantial changes in their intestine's intestinal proteome and their physiological properties. One of the most striking effects was the strong induction of lysozyme gene expression in the intestine, which was also matched by increased lysozyme activity. This effect was organ-autonomous, as it was also observed in flies in which dSirt4 was knocked down only in the intestinal enterocytes. This significantly increased lysozyme expression in response to dSirt4 downregulation did not reduce the total number of bacteria in the gut, but rather shifted the composition of the microbiota by reducing the number of gram-positive bacteria. This effect on microbiota composition can be attributed to dSirt4-dependent lysozyme expression, as it is absent in a lysozyme-deficient background. dSirt4 deficiency in the enterocytes shortened the lifespan of the flies, which was also observed in flies with ectopic lysozyme overexpression in the enterocytes. We assume that high lysozyme expression leads to a dysbiotic state of the microbiota associated with a shortened lifespan.
HostingRepository	PRIDE
AnnounceDate	2025-06-23
AnnouncementXML	Submission_2025-06-22_16:20:54.659.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andreas Tholey
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-08 04:24:17	ID requested
⏵ 1	2025-06-22 16:20:55	announced

10.1186/s42523-025-00431-x;

Knop M, Treitz C, Bettendorf S, Bossen J, von Frieling J, Doms S, Saboukh A, Bruchhaus I, K, ü, hnlein RP, Baines JF, Tholey A, Roeder T, Mitochondrial sirtuin 4 shapes the intestinal microbiota of Drosophila by controlling lysozyme expression. Anim Microbiome, 7(1):63(2025) [pubmed]

submitter keyword: Drosophila

LC-MS

sirtuin-4

Andreas Tholey
contact affiliation	Systematische Proteomics & Bioanalytik - Institut für Experimentelle Medizin Christian-Albrechts-Universität zu Kiel
contact email	a.tholey@iem.uni-kiel.de
lab head
Andreas Tholey
contact affiliation	Systematic Proteome Research & Bioanalytics, University of Kiel
contact email	a.tholey@iem.uni-kiel.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054704
     1. Label: PRIDE project
     2. Name: A mitochondrial sirtuin shapes the intestinal microbiota by controlling lysozyme expression