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PXD054703

PXD054703 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleIdentification of a FimA protein in a crude lysate SDS-PAGE gel of a P. gingivalis heme-uptake mutant
DescriptionPorphyromonas gingivalis is one of the main etiological agents responsible for initiating and progressing periodontal diseases in humans. P. gingivalis does not synthesize heme, and it uses host hemoproteins as a source of this molecule. The main heme uptake mechanism utilized by P. gingivalis is the Hmu system composed of six proteins where HmuY (hemophore-like protein) and HmuR (TonB-dependant outer membrane heme receptor) play a crucial role in heme supply. The second heme uptake mechanism is the Hus system composed of four proteins, with HusA (hemophore-like protein) and HusB (TonB-dependant outer membrane receptor), having a supporting role in heme uptake. Heme and iron starvation influence the P. gingivalis phenotype, therefore the aim was to determine the influence of deletion of HmuY and HmuR (ΔhmuYR strain) or HusA and HusB (ΔhusAB strain) encoding genes on P. gingivalis phenotype including the impact on protein production in response to the analyzed deletion. The mutant strains were constructed in wild-type A7436 P. gingivalis strain. Bacteria were grown in basal medium (BM) supplemented with 7.7 µM heme. After analysis of bacteria lysates with SDS-PAGE and CBB G-250 staining, in ΔhmuYR a protein (~45 kDa) was detected produced in higher amounts, than in the wild-type and the ΔhusAB strains. The gel band of interest, as well as its corresponding sections in the WT lanes were excised for bottom-up LC-MS analysis, and the protein was identified as FimA.
HostingRepositoryPRIDE
AnnounceDate2024-12-28
AnnouncementXMLSubmission_2024-12-28_14:42:26.352.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD054703
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMichał Tracz
SpeciesList scientific name: Porphyromonas gingivalis A7436; NCBI TaxID: 1403337;
ModificationListNo PTMs are included in the dataset
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-08 04:13:52ID requested
12024-12-28 14:42:26announced
Publication List
10.6019/PXD054703;
10.1038/S41598-024-82326-6;
Keyword List
submitter keyword: pili, SDS-PAGE, gel band analysis, pilus, bottom-up, heme-uptake mutant, MSE, hmuR,LC-MS, major fimbrium, FimA, hmuY, Porphyromonas gingivalis
Contact List
Michal Tracz
contact affiliationLaboratory of Protein Mass Spectrometry, Faculty of Biotechnology, University of Wroclaw
contact emailmichal.tracz2@uwr.edu.pl
lab head
Michał Tracz
contact affiliationLaboratory of Protein Mass Spectrometry, Faculty of Biotechnology, University of Wroclaw
contact emailmichal.tracz2@uwr.edu.pl
dataset submitter
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Dataset FTP location
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