PXD054695

PXD054695 is an original dataset announced via ProteomeXchange.

Title	Data to support: SUMO4 promotes SUMO deconjugation required for DNA double-strand break repair
Description	The amplitudes of small-modifier protein signalling through ubiquitin and the Small Ubiquitin-like Modifiers, SUMO1-3, are critical to the correct phasing of repair protein accumulation, activity and clearance, and the completion of mammalian DNA double-strand break (DSB) repair. However, how SUMO-conjugate signalling in the response is delineated is poorly understood. At the same time, the role of the non-conjugated SUMO protein, SUMO4, has remained enigmatic. The data provides support of the differential presence of SUMO4 protein in different cell lines. Together with the data in the manuscript, the work reveals that SUMO4 is required to prevent excessive DNA-damage-induced SUMOylation and deleterious over-accumulation of RAP80. Mechanistically, the data shows SUMO4 acts independently of its conjugation and potentiates SENP1 catalytic activity. Together the data identify SUMO4 as a novel SUMO deconjugation component and that SUMO4:SENP1 are critical regulators of DNA-damage-induced SUMO signalling.
HostingRepository	PRIDE
AnnounceDate	2025-03-11
AnnouncementXML	Submission_2025-03-11_05:28:31.583.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD054695
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Aneika Leney
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-08 00:18:58	ID requested
⏵ 1	2025-03-11 05:28:31	announced

10.6019/PXD054695;

10.1016/J.MOLCEL.2025.02.004;

submitter keyword: SUMO, DNA repair, proteomics data

Aneika C. Leney
contact affiliation	School of Biosciences, University of Birmingham, UK
contact email	a.leney@bham.ac.uk
lab head
Aneika Leney
contact affiliation	University of Birmingham
contact email	a.leney@bham.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054695
     1. Label: PRIDE project
     2. Name: Data to support: SUMO4 promotes SUMO deconjugation required for DNA double-strand break repair