PXD054684 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Blue Native PAGE co-fractionation of purified human endosomes |
Description | EndoMAP aims to systematically dissect the human endosomal protein complexes. To purify endosomes from human cells, a modified Endo-IP protocol was developed to be compatible with complexomics methodologies and evaluated by DIA-MS. Three independent biological replicates of human endosomes purified by the modified Endo-IP protocol were subjected to Blue Native PolyAcrylamide Gel Electrophoresis (BN-PAGE) co-fractionation to identify possible protein complexes based on correlation profiling. |
HostingRepository | PRIDE |
AnnounceDate | 2025-05-30 |
AnnouncementXML | Submission_2025-05-30_01:57:58.896.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Miguel A. Gonzalez-Lozano |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-08-07 17:05:59 | ID requested | |
1 | 2025-03-29 12:53:53 | announced | |
⏵ 2 | 2025-05-30 01:57:59 | announced | 2025-05-30: Updated project metadata. |
Publication List
Keyword List
submitter keyword: co-fractionation, Human, Endosomes, BN-PAGE,EndoMAP |
Contact List
J. Wade Harper |
contact affiliation | Department of Cell Biology, Harvard Medical School, Boston MA |
contact email | wade_harper@hms.harvard.edu |
lab head | |
Miguel A. Gonzalez-Lozano |
contact affiliation | Department of Cell Biology, Harvard Medical School, Boston MA |
contact email | miguel_gonzalezlozano@hms.harvard.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD054684 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD054684
- Label: PRIDE project
- Name: Blue Native PAGE co-fractionation of purified human endosomes