PXD054684

PXD054684 is an original dataset announced via ProteomeXchange.

Title	Blue Native PAGE co-fractionation of purified human endosomes
Description	EndoMAP aims to systematically dissect the human endosomal protein complexes. To purify endosomes from human cells, a modified Endo-IP protocol was developed to be compatible with complexomics methodologies and evaluated by DIA-MS. Three independent biological replicates of human endosomes purified by the modified Endo-IP protocol were subjected to Blue Native PolyAcrylamide Gel Electrophoresis (BN-PAGE) co-fractionation to identify possible protein complexes based on correlation profiling.
HostingRepository	PRIDE
AnnounceDate	2025-05-30
AnnouncementXML	Submission_2025-05-30_01:57:58.896.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Miguel A. Gonzalez-Lozano
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-07 17:05:59	ID requested
1	2025-03-29 12:53:53	announced
⏵ 2	2025-05-30 01:57:59	announced	2025-05-30: Updated project metadata.

10.1038/S41586-025-09059-Y;

submitter keyword: co-fractionation, Human, Endosomes, BN-PAGE,EndoMAP

J. Wade Harper
contact affiliation	Department of Cell Biology, Harvard Medical School, Boston MA
contact email	wade_harper@hms.harvard.edu
lab head
Miguel A. Gonzalez-Lozano
contact affiliation	Department of Cell Biology, Harvard Medical School, Boston MA
contact email	miguel_gonzalezlozano@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD054684

PRIDE project URI

• PRIDE
  1. PXD054684
     1. Label: PRIDE project
     2. Name: Blue Native PAGE co-fractionation of purified human endosomes