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PXD054684

PXD054684 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBlue Native PAGE co-fractionation of purified human endosomes
DescriptionEndoMAP aims to systematically dissect the human endosomal protein complexes. To purify endosomes from human cells, a modified Endo-IP protocol was developed to be compatible with complexomics methodologies and evaluated by DIA-MS. Three independent biological replicates of human endosomes purified by the modified Endo-IP protocol were subjected to Blue Native PolyAcrylamide Gel Electrophoresis (BN-PAGE) co-fractionation to identify possible protein complexes based on correlation profiling.
HostingRepositoryPRIDE
AnnounceDate2025-05-30
AnnouncementXMLSubmission_2025-05-30_01:57:58.896.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMiguel A. Gonzalez-Lozano
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-07 17:05:59ID requested
12025-03-29 12:53:53announced
22025-05-30 01:57:59announced2025-05-30: Updated project metadata.
Publication List
10.1038/S41586-025-09059-Y;
Keyword List
submitter keyword: co-fractionation, Human, Endosomes, BN-PAGE,EndoMAP
Contact List
J. Wade Harper
contact affiliationDepartment of Cell Biology, Harvard Medical School, Boston MA
contact emailwade_harper@hms.harvard.edu
lab head
Miguel A. Gonzalez-Lozano
contact affiliationDepartment of Cell Biology, Harvard Medical School, Boston MA
contact emailmiguel_gonzalezlozano@hms.harvard.edu
dataset submitter
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Dataset FTP location
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PRIDE project URI
Repository Record List
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