PXD054682 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Enrichment of Cysteine S-palmitoylated peptides using Sodium Deoxycholate Acid Precipitation - SDC-ACE |
| Description | S-palmitoylation is a poorly understood post-translational modification that is gaining more attention as an essential regulator of cellular processes. The reversible nature of S-palmitoylation allows for fine-tuned control of cellular events and adaptation to stimuli. The enrichment of S-palmitoylated proteins and peptides includes the Acyl-Biotin Exchange (ABE) method, Acyl resin-assisted Capture (Acyl-RAC), metabolic labelling, and derivatives thereof. We present a novel method of enrichment of S-palmitoylated peptides termed SDC Acid Precipitation Enrichment (SDC-ACE). Here, S-palmitoylated peptides are enriched by taking advantage of their co-precipitation with Sodium-Deoxycholate (SDC) under acidic conditions, allowing easy and fast separation of lipidated peptides from the sample suspension. We applied our novel method for the characterization of the mouse brain, providing an in-depth analysis of S-palmitoylation events within the brain and comprehensive profile of the mouse brain S-palmitoylome. Furthermore, we applied our method for mapping mouse tissue-specific S-palmitoylation, highlighting the extensive role of S-palmitoylation throughout various organs in the body. Finally, we applied our methods for studying the brain palmitoylome of diabetic db/db mouse, uncovering alterations in the palmitoylation related to obesity and type 2 diabetes. The SDC-ACE method allows fast and easy enrichment of S-palmitoylated peptides, providing a valuable tool for exploring the dynamics and function of S-palmitoylation in diverse biological systems. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-02-16 |
| AnnouncementXML | Submission_2026-02-16_03:49:01.456.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Martin R. Larsen |
| SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: NEWT:10090; scientific name: Acyrthosiphon pisum (Pea aphid); NCBI TaxID: NEWT:7029; |
| ModificationList | palmitoylated residue |
| Instrument | Orbitrap Eclipse; Orbitrap Exploris 480 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2024-08-07 13:15:40 | ID requested | |
| ⏵ 1 | 2026-02-16 03:49:02 | announced | |
Publication List
| Jensen PT, Palmisano G, Rhodes CJ, Larsen MR, Enrichment of Cysteine S-palmitoylated Peptides Using Sodium Deoxycholate Acid Precipitation. Mol Cell Proteomics, 25(2):101218(2026) [pubmed] |
| 10.1016/j.mcpro.2025.101218; |
Keyword List
| submitter keyword: Cysteine palmitoylation |
| Mouse brain palmitoylome |
| dbdb mice |
| mouse tissue specific palmitoylation |
Contact List
| Martin R. Larsen |
|---|
| contact affiliation | Department of Biochemistry and Molecular Biology University of Southern Denmark, Odense, Denmark |
| contact email | mrl@bmb.sdu.dk |
| lab head | |
| Martin R. Larsen |
|---|
| contact affiliation | University of Southern Denmark |
| contact email | mrl@bmb.sdu.dk |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
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| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD054682
- Label: PRIDE project
- Name: Enrichment of Cysteine S-palmitoylated peptides using Sodium Deoxycholate Acid Precipitation - SDC-ACE
