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PXD054667

PXD054667 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMLX phosphorylation is an evolutionarily conserved mechanism to promote lipid storage and sugar tolerance
DescriptionTo cope with food scarcity, animals store energy as lipids, which are produced from carbohydrates via de novo lipogenesis (DNL). The carbohydrate-responsive ChREBP-MLX complex is a master transcriptional regulator for DNL and lipid storage. However, our understanding of molecular mechanisms, particularly signaling pathways, controlling ChREBP-MLX complex remains incomplete. Here, we show that mammalian MLX is phosphorylated on an evolutionarily conserved motif and that MLX phosphorylation is necessary for ChREBP-MLX transcriptional activity. In Drosophila, MLX phosphorylation is essential for lipid storage and sugar tolerance during fly development. Furthermore, we identified CK2 as an MLX kinase. Although glucose phosphorylation is required for ChREBP-MLX activity, accumulation of glucose-6-phosphate (G6P) inhibits CK2-mediated MLX phosphorylation and thereby ChREBP-MLX activity. We propose that the evolutionarily conserved MLX phosphorylation is a rheostat that adjusts ChREBP-MLX activity in response to the carbohydrate availability ultimately controlling energy homeostasis.
HostingRepositoryPRIDE
AnnounceDate2025-03-13
AnnouncementXMLSubmission_2025-03-13_01:57:41.526.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD054667
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterSebastien Carpentier
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; deamidated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-07 02:57:55ID requested
12025-03-13 01:57:41announced
Publication List
10.6019/PXD054667;
10.1126/SCIADV.ADT4548;
Keyword List
submitter keyword: Carbohydrate and lipid metabolism, sugar tolerance, fly development
Contact List
Sebastien Carpentier
contact affiliationSyBioMa KULeuven
contact emailsebastien.carpentier@kuleuven.be
lab head
Sebastien Carpentier
contact affiliationKULeuven
contact emailsebastien.carpentier@kuleuven.be
dataset submitter
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Dataset FTP location
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