PXD054667

PXD054667 is an original dataset announced via ProteomeXchange.

Title	MLX phosphorylation is an evolutionarily conserved mechanism to promote lipid storage and sugar tolerance
Description	To cope with food scarcity, animals store energy as lipids, which are produced from carbohydrates via de novo lipogenesis (DNL). The carbohydrate-responsive ChREBP-MLX complex is a master transcriptional regulator for DNL and lipid storage. However, our understanding of molecular mechanisms, particularly signaling pathways, controlling ChREBP-MLX complex remains incomplete. Here, we show that mammalian MLX is phosphorylated on an evolutionarily conserved motif and that MLX phosphorylation is necessary for ChREBP-MLX transcriptional activity. In Drosophila, MLX phosphorylation is essential for lipid storage and sugar tolerance during fly development. Furthermore, we identified CK2 as an MLX kinase. Although glucose phosphorylation is required for ChREBP-MLX activity, accumulation of glucose-6-phosphate (G6P) inhibits CK2-mediated MLX phosphorylation and thereby ChREBP-MLX activity. We propose that the evolutionarily conserved MLX phosphorylation is a rheostat that adjusts ChREBP-MLX activity in response to the carbohydrate availability ultimately controlling energy homeostasis.
HostingRepository	PRIDE
AnnounceDate	2025-03-13
AnnouncementXML	Submission_2025-03-13_01:57:41.526.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD054667
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Sebastien Carpentier
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-07 02:57:55	ID requested
⏵ 1	2025-03-13 01:57:41	announced

10.6019/PXD054667;

10.1126/SCIADV.ADT4548;

submitter keyword: Carbohydrate and lipid metabolism, sugar tolerance, fly development

Sebastien Carpentier
contact affiliation	SyBioMa KULeuven
contact email	sebastien.carpentier@kuleuven.be
lab head
Sebastien Carpentier
contact affiliation	KULeuven
contact email	sebastien.carpentier@kuleuven.be
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054667
     1. Label: PRIDE project
     2. Name: MLX phosphorylation is an evolutionarily conserved mechanism to promote lipid storage and sugar tolerance