PXD054661

PXD054661 is an original dataset announced via ProteomeXchange.

Title	Phosphatidylcholine editing is critical to lipid homeostasis in Arabidopsis trigalactosyldiacylglycerol1 mutants
Description	Lipid remodeling is crucial for plant responses to abiotic stress and metabolic disturbances. A key aspect of this process involves the modification of phosphatidylcholine (PC) acyl chains by lysophosphatidylcholine: acyl-CoA acyltransferases (LPCATs). To understand their role in lipid homeostasis, we studied the trigalactosyldiacylglycerol1 (tgd1) mutant, which exhibits significant increases in fatty acid synthesis and flux through PC due to disrupted inter-organelle lipid trafficking. We discovered that the elevated fatty acid synthesis in tgd1 is due to the posttranslational activation of plastidic acetyl-coenzyme A carboxylase (ACCase). Global transcriptomic profiling revealed 62 significantly upregulated genes in tgd1, with 27 (44%) involved in RNA and DNA modifications, suggesting a role for posttranscriptional mechanisms in adapting to lipid trafficking disruptions. Additionally, transcript levels of LPCATs and several genes regulating ACCase were moderately increased in tgd1. Genetic analysis showed that knocking out LPCAT1 and LPCAT2 was lethal in the tgd1 background. Furthermore, plants homozygous for lpcat2 and heterozygous for lpcat1 in the tgd1 background displayed reduced levels of PC and TAG, along with altered fatty acid compositions. Our findings provide mechanistic insights into fatty acid synthesis regulation and highlight the critical role of LPCATs in maintaining cellular lipid homeostasis when fatty acid production exceeds the cellular demand for membrane lipid synthesis.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:58:33.189.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Saroj Sah
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	No PTMs are included in the dataset
Instrument	TSQ Altis Plus

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-07 01:50:37	ID requested
1	2024-09-16 09:12:54	announced
⏵ 2	2024-10-22 06:58:33	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: trigalactosyldiacylglycerol1, lipid homeostasis,Arabidopsis

Chancheng Xu
contact affiliation	Biology Department, Brookhaven National Laboratory, USA
contact email	cxu@bnl.gov
lab head
Saroj Sah
contact affiliation	Brookhaven National Laboratory
contact email	saroj1021@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD054661

PRIDE project URI

• PRIDE
  1. PXD054661
     1. Label: PRIDE project
     2. Name: Phosphatidylcholine editing is critical to lipid homeostasis in Arabidopsis trigalactosyldiacylglycerol1 mutants