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PXD054661

PXD054661 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePhosphatidylcholine editing is critical to lipid homeostasis in Arabidopsis trigalactosyldiacylglycerol1 mutants
DescriptionLipid remodeling is crucial for plant responses to abiotic stress and metabolic disturbances. A key aspect of this process involves the modification of phosphatidylcholine (PC) acyl chains by lysophosphatidylcholine: acyl-CoA acyltransferases (LPCATs). To understand their role in lipid homeostasis, we studied the trigalactosyldiacylglycerol1 (tgd1) mutant, which exhibits significant increases in fatty acid synthesis and flux through PC due to disrupted inter-organelle lipid trafficking. We discovered that the elevated fatty acid synthesis in tgd1 is due to the posttranslational activation of plastidic acetyl-coenzyme A carboxylase (ACCase). Global transcriptomic profiling revealed 62 significantly upregulated genes in tgd1, with 27 (44%) involved in RNA and DNA modifications, suggesting a role for posttranscriptional mechanisms in adapting to lipid trafficking disruptions. Additionally, transcript levels of LPCATs and several genes regulating ACCase were moderately increased in tgd1. Genetic analysis showed that knocking out LPCAT1 and LPCAT2 was lethal in the tgd1 background. Furthermore, plants homozygous for lpcat2 and heterozygous for lpcat1 in the tgd1 background displayed reduced levels of PC and TAG, along with altered fatty acid compositions. Our findings provide mechanistic insights into fatty acid synthesis regulation and highlight the critical role of LPCATs in maintaining cellular lipid homeostasis when fatty acid production exceeds the cellular demand for membrane lipid synthesis.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:58:33.189.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSaroj Sah
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListNo PTMs are included in the dataset
InstrumentTSQ Altis Plus
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-07 01:50:37ID requested
12024-09-16 09:12:54announced
22024-10-22 06:58:33announced2024-10-22: Updated project metadata.
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: trigalactosyldiacylglycerol1, lipid homeostasis,Arabidopsis
Contact List
Chancheng Xu
contact affiliationBiology Department, Brookhaven National Laboratory, USA
contact emailcxu@bnl.gov
lab head
Saroj Sah
contact affiliationBrookhaven National Laboratory
contact emailsaroj1021@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
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