PXD054645

PXD054645 is an original dataset announced via ProteomeXchange.

Title	Consumption of traditional Sardinian fermented milk promotes changes in the rat gut microbiota composition and functions
Description	Introduction: Fermented milk products are part of the staple diet for many Mediterranean populations. Most of these traditional foods are enriched with lactobacilli and other lactic acid bacteria and metabolites resulting from lactose fermentation. There is currently very little scientific knowledge on the influence of diet supplementation with fermented milk on the gut microbiota metabolism and composition. Methods: We integrated 16S rRNA gene-based taxonomic profiling with metaproteomic-based functional analysis to investigate the gut microbiota modifications in rats exposed for 8 weeks to diet supplementation with casu axedu, a traditional fermented milk produced within rural communities in Sardinia (Italy). Results and Discussion: Several taxa showed a significantly increased abundance at the end of the dietary treatment, including Phascolarctobacterium, Prevotella, Blautia glucerasea, and Akkermansia muciniphila, while Bifidobacterium, Lachnoclostridium, Odoribacter, Bacteroides dorei and Dubosiella newyorkensis were decreased compared to the control rats. Metaproteomic analysis highlighted a striking reshape of the Prevotella proteome profile in agreement with its blooming in casu axedu-fed animals, suggesting an increase of the glycolytic activity through the Embden Meyerhof Parnas Pathway over the Entner Doudoroff Pathway. Moreover, an increased production of succinate was observed, which in turn significantly boosted the abundance of Phascolartcobacterium and its production of propionate. Fermented milk consumption was also associated with promotion of microbial synthesis of branched chain essential amino acids L-valine and L-leucine. Finally, metaproteomic data indicated a reduction of bacterial virulence factors and host inflammatory markers, suggesting that the consumption of casu axedu can have beneficial effects on the gut mucosa health.
HostingRepository	PRIDE
AnnounceDate	2025-09-29
AnnouncementXML	Submission_2025-09-28_17:56:51.371.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Antonio Palomba
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; scientific name: rat gut metagenome; NCBI TaxID: 1436733;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-06 10:03:13	ID requested
⏵ 1	2025-09-28 17:56:52	announced

Abbondio M, Palomba A, Serra M, Fraumene C, Di Meo C, Marongiu F, Sau R, Pagnozzi D, Laconi E, Tanca A, Uzzau S, Consumption of traditional Sardinian fermented milk promotes changes in the rat gut microbiota composition and functions. BMC Microbiol, 25(1):578(2025) [pubmed]

10.1186/s12866-025-04333-5;

submitter keyword: Prevotella, metagenomics, propanoate metabolism,fermented milk, metaproteomics

Daniela Pagnozzi
contact affiliation	Proteomics and Immunodiagnostics Laboratory, Porto Conte Ricerche s.r.l., Alghero, Italy
contact email	pagnozzi@portocontericerche.it
lab head
Antonio Palomba
contact affiliation	Porto Conte Ricerche
contact email	palomba@portocontericerche.it
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054645
     1. Label: PRIDE project
     2. Name: Consumption of traditional Sardinian fermented milk promotes changes in the rat gut microbiota composition and functions