PXD054608

PXD054608 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	System-wide profiling of lysine 5-hydroxylation
Description	Lysine 5-hydroxylation (5-Hyl) has been well recognized as an essential protein post-translational modification regulating cellular structural stability, RNA alternative splicing and epigenetic gene expression. System-wide enrichment and quantification of 5-Hyl targets have been challenging due to its chemical inert nature and difficulties in differentiating structural isomers in a complex biological sample. Here, we report the development of an efficient chemical proteomic workflow for affinity enrichment and constitutional isomer specific profiling of endogenous 5-Hyl substrates based on highly selective periodate chemistry.
HostingRepository	PRIDE
AnnounceDate	2025-04-04
AnnouncementXML	Submission_2025-04-04_08:25:26.239.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yue Chen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue
Instrument	Orbitrap Fusion Lumos

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-05 14:05:49	ID requested
⏵ 1	2025-04-04 08:25:27	announced

Publication List

Sin YC, Park M, Griffin TJ, Yong J, Chen Y, A constitutional isomer selective chemical proteomic strategy for system-wide profiling of protein lysine 5-hydroxylation. Chem Sci, 15(44):18395-404(2024) [pubmed]
10.1039/d4sc05397d;

Sin YC, Park M, Griffin TJ, Yong J, Chen Y, A constitutional isomer selective chemical proteomic strategy for system-wide profiling of protein lysine 5-hydroxylation. Chem Sci, 15(44):18395-404(2024) [pubmed]

10.1039/d4sc05397d;

Keyword List

submitter keyword: chemical proteomics,lysine hydroxylation

submitter keyword: chemical proteomics,lysine hydroxylation

Contact List

Yue Chen
contact affiliation	University of Minnesota at Twin Cities
contact email	yuechen@umn.edu
lab head
Yue Chen
contact affiliation	University of Minnesota
contact email	ychen2001@yahoo.com
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD054608
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/04/PXD054608

PRIDE project URI

Repository Record List

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