PXD054584

PXD054584 is an original dataset announced via ProteomeXchange.

Title	Unveiling Transcriptional Mechanisms of B7-H3 in Breast Cancer Stem Cells Through Proteomic Approaches
Description	B7-H3, an immune checkpoint molecule, is overexpressed in various solid cancers, correlating with negative prognosis and poor clinical outcomes. However, its regulatory mechanisms in aggressive cancers and cancer stem cells (CSCs), which contribute to cancer formation, progression, chemoresistance, and recurrence, remain unclear. To elucidate the mechanisms regulating B7-H3 expression in breast CSCs, we employed DNA affinity purification-mass spectrometry (DAP-MS) with presumptive promoter regions of B7-H3 and identified several proteins binding to that promoter regions in CSC specific manner. Subsequent validation studies identified several potential transcription factors, including DNA damage-binding protein 1 (DDB1), XRCC5, PARP1, RPA1, and RPA3, for their significant impact on B7-H3 expression. In vitro inhibition of DDB1 with its known inhibitor, nitazoxanide, resulted in a marked decrease in B7-H3 expression, suppressing tumor sphere formation and cell migration in breast CSCs. Our findings reveal a transcription factor-mediated regulatory mechanism for B7-H3 in CSCs through proteomic analysis and highlight the critical role of these transcription factors in regulating B7-H3 expression. These results may elucidate underlying mechanisms, paving the way for the development of new immunotherapeutic strategies against breast cancer.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_23:49:39.202.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD054584
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yuri Seo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-05 02:42:47	ID requested
⏵ 1	2025-05-06 23:49:40	announced

Seo YR, Kim HB, Jung H, Kim EG, Huh S, Yi EC, Kim KM, Unveiling transcriptional mechanisms of B7-H3 in breast cancer stem cells through proteomic approaches. iScience, 28(4):112218(2025) [pubmed]

10.1016/j.isci.2025.112218;

10.6019/PXD054584;

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project

submitter keyword: B7-H3, Cancer stem cells, Transcription factors, LC-MS/MS

Eugene C.
contact affiliation	Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National University, Republic of Korea
contact email	euyi@snu.ac.kr
lab head
Yuri Seo
contact affiliation	Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology and College of Medicine or College of Pharmacy, Seoul National University, Republic of Korea
contact email	yuri.seo@snu.ac.kr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD054584

PRIDE project URI

• PRIDE
  1. PXD054584
     1. Label: PRIDE project
     2. Name: Unveiling Transcriptional Mechanisms of B7-H3 in Breast Cancer Stem Cells Through Proteomic Approaches