PXD054562

PXD054562 is an original dataset announced via ProteomeXchange.

Title	Unraveling molecular and functional responses across three lung injury models to expand the donor lung pool
Description	Background: Lung transplantation remains hampered by a scarcity of viable donor lungs, partially attributed to donor lung injuries. Methods: Three porcine lung injury models were studied: infection-induced using lipopolysaccharide (LPS) (n=7), aspiration-induced using endotracheal gastric content (n=7), and injury using lavage and harmful ventilation (VILI) (n=7). Molecular and functional changes from before and after the establishment of lung injury were examined with histopathology, immunohistochemistry, cytokine levels, hemodynamics, and mass spectrometric analysis of lung tissue. The respiratory tract lining fluid was analyzed using exhaled breath particles. Results: T-cell proliferation and suppression of complement activation were unique to the gastric injury, while the VILI group displayed a unique activation of monocyte chemotaxis. The LPS injury exhibited an activation of stress response proteins. Alterations in the extracellular matrix, particularly the degradation of collagen type IV and increased elastin expression were identified as a consistent indicator of acute lung injury. Additionally, increases in exhaled particles and differential expression of proteins in the RTLF correlated with deteriorating lung function. Conclusions: Molecular analysis of the lung indicated distinct key differences and similarities of donor lung injury phenotypes. Analysis of various donor lung injuries suggests a heightened emphasis on the ECM for the restoration and rejuvenation of damaged donor lungs.
HostingRepository	PRIDE
AnnounceDate	2025-06-30
AnnouncementXML	Submission_2025-06-29_17:08:32.306.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gabriel Hirdman
SpeciesList	scientific name: Sus scrofa domesticus (domestic pig); NCBI TaxID: 9825;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-04 11:40:00	ID requested
⏵ 1	2025-06-29 17:08:33	announced

10.1097/tp.0000000000005353;

Hirdman G, Stenlo M, B, è, chet NB, Niroomand A, Mittendorfer M, Wang Q, Edstr, ö, m D, Ghaidan H, Kjellstr, ö, m S, Pierre L, Olm F, Hyll, é, n S, Lindstedt S, Unraveling Molecular and Functional Responses Across 3 Lung Injury Models to Expand the Donor Lung Pool. Transplantation, 109(7):1166-1174(2025) [pubmed]

submitter keyword: Lung Transplantation, EBP,LC-MSMS

Sandra Lindstedt
contact affiliation	Lund University
contact email	sandra.lindstedt@med.lu.se
lab head
Gabriel Hirdman
contact affiliation	Lund University
contact email	gabriel.hirdman@med.lu.se
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054562
     1. Label: PRIDE project
     2. Name: Unraveling molecular and functional responses across three lung injury models to expand the donor lung pool