PXD054538

PXD054538 is an original dataset announced via ProteomeXchange.

Title	Conserved hydrophilic checkpoints tune FocA-1 mediated formate:H+ symport
Description	FocA belongs to the widespread, evolutionarily ancient formate-nitrite transporter 30 (FNT) family of pentameric anion channels and translocates formic acid bidirectionally. 31 Here, we identify compartmentalized polarity distribution across the complete FocA 32 pore structure – resolved at 2.56 Å – mirrored against a two-fold axis with H209 at its 33 center. The FocA-H209N efflux-only variant reveals a density consistent with formic 34 acid located directly at N209, breaking local polarity distribution. Pyruvate formate-35 lyase, generating formate, orients at the cytoplasmic face where formate delivery is 36 regulated by conformational changes in the FocA vestibule. Comparisons with other 37 FNTs suggest a tuning mechanism of formate-specific transport via checkpoints 38 enriched in hydrophilic residues.
HostingRepository	PRIDE
AnnounceDate	2025-11-10
AnnouncementXML	Submission_2025-11-09_16:06:32.578.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Christian Ihling
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: NEWT:562;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-02 06:21:51	ID requested
⏵ 1	2025-11-09 16:06:33	announced

T, ü, ting C, Janson K, Kammel M, Ihling C, Lorenz J, Kyrilis FL, Hamdi F, Erdmann C, Sinz A, Sawers RG, Kastritis PL, symport. Nat Commun, 16(1):9476(2025) [pubmed]

10.1038/s41467-025-65159-3;

submitter keyword: FocA, XLMS, Cryo EM, formate-nitrite transporter

R. Gary Sawers
contact affiliation	Institute of Biology/ Microbiology, Martin Luther University Halle-Wittenberg, Kurt-14 Mothes-Str. 3, 06120 Halle (Saale), Germany
contact email	gary.sawers@mikrobiologie.uni-halle.de
lab head
Christian Ihling
contact affiliation	MLU Halle, Inst. f. Pharmacy
contact email	christian.ihling@pharmazie.uni-halle.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/11/PXD054538

PRIDE project URI

• PRIDE
  1. PXD054538
     1. Label: PRIDE project
     2. Name: Conserved hydrophilic checkpoints tune FocA-1 mediated formate:H+ symport