<<< Full experiment listing

PXD054535

PXD054535 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleParadiplonema papillatum CENPA-V5 IP
DescriptionTransmission of genetic material from one generation to the next is a fundamental feature of all living cells. In eukaryotes, a macromolecular complex called the kinetochore plays crucial roles in this process by providing linkage between chromosomes and spindle microtubules. Little is known about this process in evolutionarily diverse protists. Within the supergroup Discoba, Eeuglenozoans forms a highly diversespeciose group of unicellular flagellates -including kinetoplastids, euglenids, and diplonemids. Kinetoplastids have an unconventional kinetochore system, while euglenids have a canonical onekinetochore system. It remains unclear what kind of kinetochores are present in diplonemids, a group of extremely diverse and abundant marine flagellatesplankton. Here, we employed deep homology detection protocols using profile-versus-profile Hidden Markov Model searches and AlphaFold-based structural comparisons to detect homologies that might have been previously missed. Interestingly, we still could not detect orthologs for most of the kinetoplastid nor canonical kinetochore subunits with few exceptions including a putative centromere-specific histone H3 variant (cenH3/CENP-A), the spindle checkpoint protein Mad2, the chromosomal passenger complex members Aurora and INCENP, and broadly conserved proteins like the CLK kinase and the meiotic synaptonemal complex proteins SYCP2 that function at kinetoplastid kinetochores. We examined the localization of five candidate kinetochore-associated proteins in the model diplonemid, Paradiplonema papillatum. PpCENP-A shows discrete dots in the nucleus, implying that it is likely a kinetochore component. PpMad2, PpCLK, PpSYCP2L1 and INCENP reside in the nucleus, but no clear kinetochore localization was observed. Altogether, these results raise a possibility that diplonemids evolved a hitherto unknown type of kinetochore system.
HostingRepositoryPRIDE
AnnounceDate2024-10-21
AnnouncementXMLSubmission_2024-10-20_23:32:14.399.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterCorinna Benz
SpeciesList scientific name: Diplonema papillatum; NCBI TaxID: 91374;
ModificationListNo PTMs are included in the dataset
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-02 05:05:55ID requested
12024-10-20 23:32:14announced
Publication List
10.1128/MBIO.02936-24;
Keyword List
submitter keyword: Paradiplonema, histone variant, kinetochore
Contact List
Julius Lukes
contact affiliationInstitute of Parasitology, Biology Center, Czech Academy of Sciences, Branišovská 31, 370 05 České Budějovice, CZECH REPUBLIC
contact emailjula@paru.cas.cz
lab head
Corinna Benz
contact affiliationInstitute of Parasitology Biology Center, Czech Academy of Sciences Branišovská 31 370 05 České Budějovice
contact emailcorinna.benz@paru.cas.cz
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD054535
PRIDE project URI
Repository Record List
[ + ]