PXD054527 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Comparative DDA and DIA-PASEF proteomics profiling of cross-kingdom macrophage infection reveals new mechanisms driving pathogen virulence |
Description | Opportunistic infections of the respiratory tract often succeed under a weakened immune response caused by an underlying illness or hospitalization. The human fungal pathogen, Cryptococcus neoformans, and the bacterial pathogen, Klebsiella pneumoniae, are both well-characterized microbes that cause severe infections within immunocompromised individuals. In this study, we simulate a concentration-dependent pulmonary coinfection of a bacterial and fungal pathogen, and profile the proteomic changes by DDA vs. DIA. Dual perspective profiling provides new insights into host defense regulation of infection and pathogenic mechanisms of invasion. |
HostingRepository | PRIDE |
AnnounceDate | 2024-08-13 |
AnnouncementXML | Submission_2024-09-13_10:02:54.409.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Jonathan Krieger |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Klebsiella pneumoniae subsp. pneumoniae CIP 52.145 = B5055; NCBI TaxID: 1205678; scientific name: Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii); NCBI TaxID: 235443; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | timsTOF Pro |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-08-02 03:01:32 | ID requested | |
⏵ 1 | 2024-09-13 10:02:54 | announced | |
Publication List
Keyword List
submitter keyword: Cryptococcus neoformans, virulence, DIA-PASEF, Klebsiella pneumoniae, macrophages, druggable targets,Mass spectrometry-based proteomics, DDA |
Contact List
Jennifer Geddes-McAlister |
contact affiliation | Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada |
contact email | jgeddesm@uoguelph.ca |
lab head | |
Jonathan Krieger |
contact affiliation | Bruker |
contact email | JRKrieger@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD054527 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD054527
- Label: PRIDE project
- Name: Comparative DDA and DIA-PASEF proteomics profiling of cross-kingdom macrophage infection reveals new mechanisms driving pathogen virulence