PXD054527

PXD054527 is an original dataset announced via ProteomeXchange.

Title	Comparative DDA and DIA-PASEF proteomics profiling of cross-kingdom macrophage infection reveals new mechanisms driving pathogen virulence
Description	Opportunistic infections of the respiratory tract often succeed under a weakened immune response caused by an underlying illness or hospitalization. The human fungal pathogen, Cryptococcus neoformans, and the bacterial pathogen, Klebsiella pneumoniae, are both well-characterized microbes that cause severe infections within immunocompromised individuals. In this study, we simulate a concentration-dependent pulmonary coinfection of a bacterial and fungal pathogen, and profile the proteomic changes by DDA vs. DIA. Dual perspective profiling provides new insights into host defense regulation of infection and pathogenic mechanisms of invasion.
HostingRepository	PRIDE
AnnounceDate	2024-08-13
AnnouncementXML	Submission_2024-09-13_10:02:54.409.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jonathan Krieger
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Klebsiella pneumoniae subsp. pneumoniae CIP 52.145 = B5055; NCBI TaxID: 1205678; scientific name: Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii); NCBI TaxID: 235443;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-02 03:01:32	ID requested
⏵ 1	2024-09-13 10:02:54	announced

10.1021/ACS.JPROTEOME.4C00255;

submitter keyword: Cryptococcus neoformans, virulence, DIA-PASEF, Klebsiella pneumoniae, macrophages, druggable targets,Mass spectrometry-based proteomics, DDA

Jennifer Geddes-McAlister
contact affiliation	Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada
contact email	jgeddesm@uoguelph.ca
lab head
Jonathan Krieger
contact affiliation	Bruker
contact email	JRKrieger@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD054527

PRIDE project URI

• PRIDE
  1. PXD054527
     1. Label: PRIDE project
     2. Name: Comparative DDA and DIA-PASEF proteomics profiling of cross-kingdom macrophage infection reveals new mechanisms driving pathogen virulence