⮝ Full datasets listing

PXD054527

PXD054527 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative DDA and DIA-PASEF proteomics profiling of cross-kingdom macrophage infection reveals new mechanisms driving pathogen virulence
DescriptionOpportunistic infections of the respiratory tract often succeed under a weakened immune response caused by an underlying illness or hospitalization. The human fungal pathogen, Cryptococcus neoformans, and the bacterial pathogen, Klebsiella pneumoniae, are both well-characterized microbes that cause severe infections within immunocompromised individuals. In this study, we simulate a concentration-dependent pulmonary coinfection of a bacterial and fungal pathogen, and profile the proteomic changes by DDA vs. DIA. Dual perspective profiling provides new insights into host defense regulation of infection and pathogenic mechanisms of invasion.
HostingRepositoryPRIDE
AnnounceDate2024-08-13
AnnouncementXMLSubmission_2024-09-13_10:02:54.409.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJonathan Krieger
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Klebsiella pneumoniae subsp. pneumoniae CIP 52.145 = B5055; NCBI TaxID: 1205678; scientific name: Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii); NCBI TaxID: 235443;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-08-02 03:01:32ID requested
12024-09-13 10:02:54announced
Publication List
10.1021/ACS.JPROTEOME.4C00255;
Keyword List
submitter keyword: Cryptococcus neoformans, virulence, DIA-PASEF, Klebsiella pneumoniae, macrophages, druggable targets,Mass spectrometry-based proteomics, DDA
Contact List
Jennifer Geddes-McAlister
contact affiliationDepartment of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario, Canada
contact emailjgeddesm@uoguelph.ca
lab head
Jonathan Krieger
contact affiliationBruker
contact emailJRKrieger@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/08/PXD054527
PRIDE project URI
Repository Record List
[ + ]