PXD054520

PXD054520 is an original dataset announced via ProteomeXchange.

Title	Transcriptomic and proteomic analysis of human brain tissue from epileptic and non-epileptic groups
Description	Abstract Objective: Epilepsy is a prevalent neurological disorder marked by seizures due to abnormal brain neuron discharge. Its pathological mechanism remains incompletely understood. This study utilizes transcriptomic and proteomic analyses on epileptic lesions from patients and brain tissue from non-epileptic individuals. The goal is to delve deeper into the molecular expression characteristics in the brain tissue of epilepsy patients, aiming to uncover potential biomarkers and therapeutic targets for epilepsy. Methods: Brain tissues from epileptic lesions post-surgical resection in epilepsy patients and brain tissues from non-epileptic patients were collected. RNA-seq transcriptomic analysis and isobaric Tags for Relative and Absolute Quantitation (iTRAQ) proteomic analysis were performed, followed by a combined analysis of transcriptomic and proteomic data. Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR), Western Blot (WB) and immunohistochemical staining (IHC) techniques were used to verify the expression levels of differentially expressed genes (DEGs) and differentially expressed proteins (DEPs). Results: Transcriptomic analysis identified 1604 differentially expressed genes, with 584 up-regulated and 1020 down-regulated. Proteomic analysis identified 694 differentially expressed proteins, with 331 up-regulated and 363 down-regulated. Combined transcriptomic and proteomic analyses showed that differential genes/proteins were mainly enriched in biological processes such as D-aspartate transport, transmembrane transport, cell junctions, vesicle transport, and metabolic processes. Tubulin Polymerization Promoting Protein Family Member 3 (TPPP3), Proprotein Convertase Subtilisin/Kexin Type 1 (PCSK1), and Dihydropyrimidinase-Like 3 (DPYSL3) were significantly altered in epilepsy patients, and their expression trends were confirmed by RT-qPCR, WB and IHC aligning with the transcriptomics and proteomics data. Significance: By integrating transcriptomic and proteomic analyses, this study identified differential genes/proteins between epileptic and non-epileptic patients and their associated biological processes. Three key differential genes/proteins, TPPP3, PCSK1, and DPYSL3, were highlighted, indicating their potential significance in the pathological mechanisms of epilepsy.
HostingRepository	iProX
AnnounceDate	2024-08-01
AnnouncementXML	Submission_2025-12-04_23:34:24.565.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Taiyang Zhu
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Astral

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-01 21:09:32	ID requested
⏵ 1	2025-12-04 23:34:25	announced

Zhu T, Zhou Y, Zhang L, Kong L, Tang H, Xiao Q, Sun X, Shen F, Zhou H, Ni W, Liu S, Gao H, Jin G, Jia X, Hua F, A transcriptomic and proteomic analysis and comparison of human brain tissue from patients with and without epilepsy. Sci Rep, 15(1):16369(2025) [pubmed]

submitter keyword: Epilepsy, Multi-omics analysis, TPPP3, PCSK1, DPYSL3

Hua Fang
contact affiliation	Affiliated Hospital of Xuzhou Medical University
contact email	huafang@xzhmu.edu.cn
lab head
Taiyang Zhu
contact affiliation	Affiliated Hospital of Xuzhou Medical University
contact email	874796495@qq.com
dataset submitter

iProX dataset URI