PXD054514

PXD054514 is an original dataset announced via ProteomeXchange.

Title	End-to-end Throughput Chemical Proteomics for Photoaffinity Labeling Target Engagement and Deconvolution
Description	Photoaffinity labeling (PAL) methodologies have proven to be a critical tool for the unbiased deconvolution of protein-ligand binding events in complex biological systems. However, like other chemical proteomic workflows, it is limited by time-intensive sample manipulations and data acquisition techniques. Here, we describe an approach to address this challenge through the innovation of a carboxylate bead-based protein cleanup procedure to isolate protein and coupling it to plate-based, proteomic sample processing as a semi-automated solution. Combining this with label-free, data-independent acquisition (DIA) sample analysis led to improvements on a workflow time per sample basis over current standard practices. This unified strategy for processing and analyzing these complex samples could greatly facilitate drug discovery efforts and open new opportunities in chemical proteomics.
HostingRepository	PRIDE
AnnounceDate	2024-10-08
AnnouncementXML	Submission_2024-10-08_07:00:58.730.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sheldon Cheung
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-01 20:19:28	ID requested
⏵ 1	2024-10-08 07:00:59	announced

10.1021/ACS.JPROTEOME.4C00442;

submitter keyword: immortalized cell lines, Orbitrap Fusion Lumos,human

Sheldon Cheung
contact affiliation	Janssen Research & Development
contact email	scheung7@its.jnj.com
lab head
Sheldon Cheung
contact affiliation	Janssen Research & Development
contact email	scheung7@its.jnj.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD054514

PRIDE project URI

• PRIDE
  1. PXD054514
     1. Label: PRIDE project
     2. Name: End-to-end Throughput Chemical Proteomics for Photoaffinity Labeling Target Engagement and Deconvolution