PXD054507

PXD054507 is an original dataset announced via ProteomeXchange.

Title	Identification of the proteins composing the CO dehydrogenase and furfurylformamide dehydrogenase enzymes purified from a thermophilic ethane-oxidizing microbial consortium
Description	Identification of the peptides composing the enriched multisubunit enzymes natively purified from the microbial enrichment, based on gel bands obtained by native electrophoresis. The anaerobic oxidation of alkanes is a microbial process occurring in deep-sea hydrocarbon seeps that plays a key ecological role in these exotic niches. The metabolic capacity of anaerobic ethane oxidation, involving uncharted biochemistry, was reported in two archaeal species depending on sulfate-reducing partner bacteria. This study deciphers the molecular basis of the CO2-generating steps of ethanotrophy by characterising the native archaeal enzymes isolated from a thermophilic enrichment. While other microorganisms couple these steps to ferredoxin reduction, we found that the CO-dehydrogenase and the formylmethanofuran-dehydrogenase are bound to F420-reductase modules. The crystal structures of these multi-metalloenzyme complexes revealed electronic bridges coupling C1-oxidation to F420-reduction. Accordingly, both systems exhibit robust F420-reductase activities, which are not detected in methanogenic or methanotrophic relative organisms. We speculate that the whole catabolism of these archaea is reoriented towards F420-reduction, which facilitates the electron transfer to the sulfate-reducing partner, therefore representing the driving force of ethanotrophy.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_06:59:18.900.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Barbara Steigenberger
SpeciesList	scientific name: Candidatus Ethanoperedens thermophilum; NCBI TaxID: 2766897;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2024-08-01 14:15:39	ID requested
1	2024-09-23 02:57:16	announced
⏵ 2	2024-10-22 06:59:19	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Anaerobic ethane oxidation, Carbon monoxide dehydrogenase, formymethanofuran dehydrogenase, multisubunit metalloenzymes, microbial metabolism

Tristan Wagner
contact affiliation	Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, 28359, Bremen, Germany
contact email	twagner@mpi-bremen.de
lab head
Barbara Steigenberger
contact affiliation	MPI of Biochemistry
contact email	steigenberger@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/09/PXD054507

PRIDE project URI

• PRIDE
  1. PXD054507
     1. Label: PRIDE project
     2. Name: Identification of the proteins composing the CO dehydrogenase and furfurylformamide dehydrogenase enzymes purified from a thermophilic ethane-oxidizing microbial consortium