PXD054505 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Transcription factor EB sets the stage for fate decisions of antigen-primed B cells in the absence and presence of co-stimulation |
Description | Ligation of the B cell antigen receptor (BCR) initiates humoral immunity. However, mere BCR signaling without appropriate co-stimulation commits B cells to death rather than to differentiation into immune effector cells. How BCR activation depletes potentially autoreactive B cells while simultaneously primes for receiving rescue and differentiation signals from cognate T lymphocytes remains unknown. Here, using a mass spectrometry-based proteomic approach to identify cytosolic/nuclear shuttling elements, we uncover transcription factor EB (TFEB) as a central BCR-controlled rheostat that drives activation-induced apoptosis, and concurrently, promotes the reception of co-stimulatory rescue signals by supporting B cell migration and antigen presentation. CD40 co-stimulation prevents TFEB-driven cell death, while enhancing and prolonging TFEB’s nuclear residency, which hallmarks antigenic experience also of memory B cells. In mice, TFEB shapes the transcriptional landscape of germinal center B cells. Within the germinal center, TFEB facilitates the dark zone entry of light-zone-residing centrocytes through regulation of chemokine receptors and, by balancing the expression of Bcl-2/BH3-only family members, integrates antigen-induced apoptosis with T cell-provided CD40 survival signals. Thus, TFEB reprograms antigen-primed germinal center B cells for cell fate decisions. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_06:52:59.052.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Christof Lenz |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-08-01 09:27:21 | ID requested | |
1 | 2024-08-08 03:41:58 | announced | |
⏵ 2 | 2024-10-22 06:52:59 | announced | 2024-10-22: Updated project metadata. |
Publication List
Keyword List
submitter keyword: B cell receptor, signal transduction,B cells, immune cell death, gene regulation in immune cells |
Contact List
Christof Lenz |
contact affiliation | Core Facility Proteomics, Department of Clinical Chemistry, University Medical Center Goettingen, Germany |
contact email | christof.lenz@med.uni-goettingen.de |
lab head | |
Christof Lenz |
contact affiliation | Max Planck Institute for Biophysical Chemistry |
contact email | christof.lenz@mpibpc.mpg.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD054505
- Label: PRIDE project
- Name: Transcription factor EB sets the stage for fate decisions of antigen-primed B cells in the absence and presence of co-stimulation