PXD054477

PXD054477 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	MBEC4 cells with low-Arginine conditions
Description	Amino acid transporters are expressed in the brain capillary endothelial cells, which form the blood-brain barrier, and their expression levels change during the neonatal period. This study aimed to investigate the molecular mechanisms regulating the amino acid transporter levels in mouse brain capillary endothelial cells. Proteomic analysis revealed arginine depletion-dependent induction of the amino acid transporters, Slc1a4, Slc3a2, Slc7a1, Slc7a5, and Slc38a1. These effects were also inhibited by GCN2iB, suggesting the involvement of eIF2K4 activation. In contrast, expression levels of Slc2a1, Slc16a1, Abcb1b, Abcg2, transferrin receptor, insulin receptor, claudin-1, Zo-1, and Jam1 were not suppressed by GCN2iB.
HostingRepository	jPOST
AnnounceDate	2025-04-11
AnnouncementXML	Submission_2025-04-10_08:00:13.759.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sumio Ohtsuki
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	S-carboxamidomethyl-L-cysteine; L-methionine sulfoxide; O-phospho-L-serine; O-phospho-L-threonine
Instrument	instrument

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-31 22:52:51	ID requested
⏵ 1	2025-04-10 08:00:15	announced

Publication List

Hamada Y, Masuda T, Ito S, Ohtsuki S, K4 in Amino Acid Transporter Expression in Mouse Brain Capillary Endothelial Cells. Pharm Res, 41(11):2213-2223(2024) [pubmed]

Hamada Y, Masuda T, Ito S, Ohtsuki S, K4 in Amino Acid Transporter Expression in Mouse Brain Capillary Endothelial Cells. Pharm Res, 41(11):2213-2223(2024) [pubmed]

Keyword List

submitter keyword: mouse, brain microvascular endothelial cells, amino acid, DIA

submitter keyword: mouse, brain microvascular endothelial cells, amino acid, DIA

Contact List

Sumio Ohtsuki
lab head
Sumio Ohtsuki
contact affiliation	Kumamoto University
dataset submitter

Full Dataset Link List

jPOST dataset URI
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jPOST dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST003235/