PXD054476

PXD054476 is an original dataset announced via ProteomeXchange.

Title	Antithrombotic coating with sheltered positive charges prevents contact activation by controlling Factor XII-biointerface binding
Description	The development of an antithrombotic surface that prevents coagulation activation without interfering with hemostasis is the “holy grail” for blood-contacting devices. Such an advance would restrain device-induced thrombogenesis, and decrease the need for anticoagulant use in conjunction with devices, which would in turn reduce the bleeding risk. Herein, by optimizing factor (F)XII-surface binding rather than preventing its surface adsorption, we developed a substrate-independent antithrombotic coating with sheltered positive charges, the function of which was verified in vitro in human blood and using a carotid artery-jugular vein shunt model in rabbits. The new coating exhibits a strong interaction with FXII but results in a low reciprocal activation of the contact pathway. These findings contradict the prevailing strategy of designing antithrombotic materials through protein-repelling surfaces. Besides providing a universal coating which can benefit most blood-contacting devices, this work also pioneers a simple, but powerful and counterintuitive, guideline for designing antithrombotic surfaces.
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_23:19:16.391.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	HAIFENG JI
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Bruker Daltonics timsTOF series

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-31 22:38:46	ID requested
⏵ 1	2025-05-06 23:19:17	announced

Ji H, Yu K, Abbina S, Xu L, Xu T, Cheng S, Vappala S, Arefi SMA, Rana MM, Chafeeva I, Drayton M, Gonzalez K, Liu Y, Grecov D, Conway EM, Zhao W, Zhao C, Kizhakkedathu JN, Antithrombotic coating with sheltered positive charges prevents contact activation by controlling factor XII-biointerface binding. Nat Mater, 24(4):626-636(2025) [pubmed]

10.1038/s41563-024-02046-0;

submitter keyword: antithrombotic coating, surface-protein interaction,Coagulation FXII

Weifeng Zhao
contact affiliation	College of Polymer Science and Engineering, State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, 610065, People's Republic of China
contact email	zhaoscukth@163.com
lab head
HAIFENG JI
contact affiliation	UBC
contact email	haifeng.ji@ubc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD054476

PRIDE project URI

• PRIDE
  1. PXD054476
     1. Label: PRIDE project
     2. Name: Antithrombotic coating with sheltered positive charges prevents contact activation by controlling Factor XII-biointerface binding