PXD054475

PXD054475 is an original dataset announced via ProteomeXchange.

Title	PNET1 is an on-demand component of the nuclear pore complex gating cell proliferation rates
Description	The nuclear pore complex (NPC) is a cornerstone of eukaryotic cell functionality, orchestrating the nucleocytoplasmic shuttling of macromolecules. Here, we report that PNET1, a transmembrane nucleoporin conserved between humans and plants, is a dynamic, adaptable NPC component, exclusively expressed in actively dividing cells. PNET1's selective integration is crucial for rapid cell growth in highly proliferative meristem and callus tissues in plants, as well as in lung cancer tissues in humans. We demonstrated that PNET1 acts as a pivotal tuner to coordinate mitotic disassembly and post-mitotic reassembly of the NPC during cell cycle. PNET1 hyper-phosphorylation disrupts its interaction with NPC scaffold, promoting NPC disintegration and nuclear membrane breakdown to trigger mitosis. Nascent, unphosphorylated PNET1 restores NPC interactions and recruits NPC scaffold for its reassembly during nuclear membrane rebuilding in daughter cells, continuing the cycle. Our findings reveal a previously uncharted mechanism that ensures rapid cell proliferation via exploiting an on-demand nucleoporin.
HostingRepository	PRIDE
AnnounceDate	2025-03-10
AnnouncementXML	Submission_2025-03-10_08:39:12.981.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD054475
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yiling Fang
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	biotinylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-31 22:35:04	ID requested
⏵ 1	2025-03-10 08:39:13	announced

10.1038/s41477-025-01908-y;

Fang Y, Tang Y, Xie P, Hsieh K, Nam H, Jia M, Reyes AV, Liu Y, Xu S, Xu X, Gu Y, Nucleoporin PNET1 coordinates mitotic nuclear pore complex dynamics for rapid cell division. Nat Plants, 11(2):295-308(2025) [pubmed]

10.6019/PXD054475;

submitter keyword: Arabidopsis thaliana, callus, nuclear pore complex

Yangnan Gu
contact affiliation	Plant & Microbial Biology, Gu lab, University of California, Berkeley, USA
contact email	guyangnan@berkeley.edu
lab head
Yiling Fang
contact affiliation	UC Berkeley
contact email	yiling_fang@berkeley.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD054475

PRIDE project URI

• PRIDE
  1. PXD054475
     1. Label: PRIDE project
     2. Name: PNET1 is an on-demand component of the nuclear pore complex gating cell proliferation rates