PXD054475 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | PNET1 is an on-demand component of the nuclear pore complex gating cell proliferation rates |
Description | The nuclear pore complex (NPC) is a cornerstone of eukaryotic cell functionality, orchestrating the nucleocytoplasmic shuttling of macromolecules. Here, we report that PNET1, a transmembrane nucleoporin conserved between humans and plants, is a dynamic, adaptable NPC component, exclusively expressed in actively dividing cells. PNET1's selective integration is crucial for rapid cell growth in highly proliferative meristem and callus tissues in plants, as well as in lung cancer tissues in humans. We demonstrated that PNET1 acts as a pivotal tuner to coordinate mitotic disassembly and post-mitotic reassembly of the NPC during cell cycle. PNET1 hyper-phosphorylation disrupts its interaction with NPC scaffold, promoting NPC disintegration and nuclear membrane breakdown to trigger mitosis. Nascent, unphosphorylated PNET1 restores NPC interactions and recruits NPC scaffold for its reassembly during nuclear membrane rebuilding in daughter cells, continuing the cycle. Our findings reveal a previously uncharted mechanism that ensures rapid cell proliferation via exploiting an on-demand nucleoporin. |
HostingRepository | PRIDE |
AnnounceDate | 2025-03-10 |
AnnouncementXML | Submission_2025-03-10_08:39:12.981.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD054475 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Yiling Fang |
SpeciesList | scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; |
ModificationList | biotinylated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2024-07-31 22:35:04 | ID requested | |
⏵ 1 | 2025-03-10 08:39:13 | announced | |
Publication List
10.1038/s41477-025-01908-y; |
Fang Y, Tang Y, Xie P, Hsieh K, Nam H, Jia M, Reyes AV, Liu Y, Xu S, Xu X, Gu Y, Nucleoporin PNET1 coordinates mitotic nuclear pore complex dynamics for rapid cell division. Nat Plants, 11(2):295-308(2025) [pubmed] |
10.6019/PXD054475; |
Keyword List
submitter keyword: Arabidopsis thaliana, callus, nuclear pore complex |
Contact List
Yangnan Gu |
contact affiliation | Plant & Microbial Biology, Gu lab, University of California, Berkeley, USA |
contact email | guyangnan@berkeley.edu |
lab head | |
Yiling Fang |
contact affiliation | UC Berkeley |
contact email | yiling_fang@berkeley.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD054475 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD054475
- Label: PRIDE project
- Name: PNET1 is an on-demand component of the nuclear pore complex gating cell proliferation rates