⮝ Full datasets listing

PXD054475

PXD054475 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitlePNET1 is an on-demand component of the nuclear pore complex gating cell proliferation rates
DescriptionThe nuclear pore complex (NPC) is a cornerstone of eukaryotic cell functionality, orchestrating the nucleocytoplasmic shuttling of macromolecules. Here, we report that PNET1, a transmembrane nucleoporin conserved between humans and plants, is a dynamic, adaptable NPC component, exclusively expressed in actively dividing cells. PNET1's selective integration is crucial for rapid cell growth in highly proliferative meristem and callus tissues in plants, as well as in lung cancer tissues in humans. We demonstrated that PNET1 acts as a pivotal tuner to coordinate mitotic disassembly and post-mitotic reassembly of the NPC during cell cycle. PNET1 hyper-phosphorylation disrupts its interaction with NPC scaffold, promoting NPC disintegration and nuclear membrane breakdown to trigger mitosis. Nascent, unphosphorylated PNET1 restores NPC interactions and recruits NPC scaffold for its reassembly during nuclear membrane rebuilding in daughter cells, continuing the cycle. Our findings reveal a previously uncharted mechanism that ensures rapid cell proliferation via exploiting an on-demand nucleoporin.
HostingRepositoryPRIDE
AnnounceDate2025-03-10
AnnouncementXMLSubmission_2025-03-10_08:39:12.981.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD054475
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterYiling Fang
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListbiotinylated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-07-31 22:35:04ID requested
12025-03-10 08:39:13announced
Publication List
10.1038/s41477-025-01908-y;
Fang Y, Tang Y, Xie P, Hsieh K, Nam H, Jia M, Reyes AV, Liu Y, Xu S, Xu X, Gu Y, Nucleoporin PNET1 coordinates mitotic nuclear pore complex dynamics for rapid cell division. Nat Plants, 11(2):295-308(2025) [pubmed]
10.6019/PXD054475;
Keyword List
submitter keyword: Arabidopsis thaliana, callus, nuclear pore complex
Contact List
Yangnan Gu
contact affiliationPlant & Microbial Biology, Gu lab, University of California, Berkeley, USA
contact emailguyangnan@berkeley.edu
lab head
Yiling Fang
contact affiliationUC Berkeley
contact emailyiling_fang@berkeley.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/03/PXD054475
PRIDE project URI
Repository Record List
[ + ]