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PXD054402

PXD054402 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleLC-MS3 analysis of Herc1-knockout mouse MLL/AF9 acute myeloid leukemia cells
DescriptionOur CRISPR-based pharmacogenomic screening identified the E3 ubiquitin ligase Herc1 as a key modulator of sensitivity to cytarabine (Ara-C) chemotherapy in the MLL/AF9 (MA) mouse AML model both in vitro and in vivo. To identify the intracellular proteins whose abundance is modulated by Herc1, we performed comparative LC-MS3 analysis of in vitro-cultured MA mouse AML cells that were transduced with either a small-guide RNA targeting Herc1 (i.e., CRISPR-mediated Herc1-knockout) or a control non-targeting small-guide RNA, at steady state or after brief exposure to Ara-C (200 nMol for 6 hours).
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_06:52:46.602.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrançois Mercier
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListTMT6plex-126 reporter+balance reagent acylated residue; acetylated residue; monohydroxylated residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-07-30 09:20:23ID requested
12024-08-08 02:28:01announced
22024-10-22 06:52:47announced2024-10-22: Updated project metadata.
Publication List
10.1182/bloodadvances.2023011540;
Jankovic M, Poon WWL, Gonzales-Losada C, Vazquez GG, Sharif-Askari B, Ding Y, Craplet-Desombre C, Ilie A, Shi J, Wang Y, Jayavelu AK, Orthwein A, Mercier F É, The E3 ubiquitin ligase Herc1 modulates the response to nucleoside analogs in acute myeloid leukemia. Blood Adv, 8(20):5315-5329(2024) [pubmed]
Keyword List
submitter keyword: Herc1, mouse, LC-MS3,Acute myeloid leukemia
Contact List
Francois Mercier
contact affiliationLady Davis Institue for Medical Research, McGill University, Canada Department of Medicine, McGill University, Canada
contact emailfrancois.mercier@mcgill.ca
lab head
François Mercier
contact affiliationMcGill University
contact emailfrancois.mercier@mcgill.ca
dataset submitter
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Dataset FTP location
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