PXD054396

PXD054396 is an original dataset announced via ProteomeXchange.

Title	The MAST kinase KIN-4 provides the Greatwall activity in C. elegans
Description	Mitotic entry is triggered by the Cyclin B-Cdk1 kinase, which phosphorylates numer-ous substrates. The accumulation of these phosphorylated substrates relies on inhib-iting the opposing PP2A-B55 phosphatase. With its asynchronous cell divisions, the C. elegans embryo is an attractive model for dissecting how the cross-talk between Cyclin B-Cdk1 and PP2A-B55 ensures cell cycle length during development. Howev-er, the mechanisms that control PP2A-B55 activity have remained elusive in C. elegans because its genome encodes no obvious homolog of the Greatwall kinase, which inhibits PP2A-B55 in other metazoans. Here, we show that the single MAST kinase KIN-4, previously associated with aging and thermotaxis phenotypes, provides the Greatwall activity in C. elegans and can functionally replace Greatwall in the het-erologous Xenopus system. Furthermore, we show that a balance between Cyclin B-Cdk1 and PP2A-B55 activity, regulated by KIN-4, is essential to ensure asynchronous cell divisions. Our findings resolve a long-standing puzzle related to the supposed absence of a Greatwall-like pathway in C. elegans and highlight a novel aspect of PP2A-B55 regulation by MAST kinases with implications in several biological pro-cesses
HostingRepository	PRIDE
AnnounceDate	2025-05-07
AnnouncementXML	Submission_2025-05-06_23:08:38.336.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guillaume CHEVREUX
SpeciesList	scientific name: Caenorhabditis elegans; NCBI TaxID: 6239;
ModificationList	methylthiolated residue; phosphorylated residue; monohydroxylated residue; deamidated residue
Instrument	timsTOF Pro 2

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-30 07:39:00	ID requested
⏵ 1	2025-05-06 23:08:39	announced

Roumbo L, Ossareh-Nazari B, Vigneron S, Stefani I, Van Hove L, Legros V, Chevreux G, Lacroix B, Castro A, Joly N, Lorca T, Pintard L, The MAST kinase KIN-4 carries out mitotic entry functions of Greatwall in C. elegans. EMBO J, 44(7):1943-1974(2025) [pubmed]

10.1038/s44318-025-00364-w;

submitter keyword: Cell Cycle, Phosphatase, C. elegans, Development, Kinase

Lionel Pintard
contact affiliation	Université Paris cité, CNRS, Institut Jacques Monod, F-75006 Paris, France
contact email	lionel.pintard@ijm.fr
lab head
Guillaume CHEVREUX
contact affiliation	Institut Jacques Monod, CNRS UMR7592
contact email	guillaume.chevreux@ijm.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD054396
     1. Label: PRIDE project
     2. Name: The MAST kinase KIN-4 provides the Greatwall activity in C. elegans