PXD054394

PXD054394 is an original dataset announced via ProteomeXchange.

Title	Interdomain-linker control conformational transitions in the SLC23 elevator transporter UraA
Description	Uptake of nucleobases and ascorbate is an essential process in all living organisms mediated by SLC23 transport proteins. These transmembrane carriers operate via the elevator alternating access mechanism and are composed of two rigid domains whose relative motion drives transport. The lack of large conformational changes within these domains suggests that the interdomain-linkers act as flexible tethers. Here, we show that interdomain-linkers are not mere tethers but have a key regulatory role in dictating the conformational space of the transporter and defining the rotation axis of the mobile transport domain. By resolving a wide inward-open conformation of the SLC23 elevator transporter UraA and combining biochemical studies using a synthetic nanobody as conformational probe with hydrogen-deuterium exchange mass spectrometry, we demonstrate that interdomain-linkers control the function of transport proteins by influencing substrate affinity and transport rate. These findings open the possibility to allosterically modulate the activity of elevator proteins by targeting their linkers.
HostingRepository	PRIDE
AnnounceDate	2024-10-17
AnnouncementXML	Submission_2024-10-17_03:37:46.388.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Langer
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562;
ModificationList	No PTMs are included in the dataset
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-30 07:25:39	ID requested
⏵ 1	2024-10-17 03:37:47	announced

Kuhn BT, Z, ö, ller J, Zimmermann I, Gemeinhardt T, Ö, zkul DH, Langer JD, Seeger MA, Geertsma ER, Interdomain-linkers control conformational transitions in the SLC23 elevator transporter UraA. Nat Commun, 15(1):7518(2024) [pubmed]

10.1038/s41467-024-51814-8;

submitter keyword: HDX-MS, E. coli, UraA

Julian D. Langer
contact affiliation	Proteomics, Max Planck Institute of Biophysics, Frankfurt am Main, Germany
contact email	julian.langer@biophys.mpg.de
lab head
Julian Langer
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@biophys.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/10/PXD054394

PRIDE project URI

• PRIDE
  1. PXD054394
     1. Label: PRIDE project
     2. Name: Interdomain-linker control conformational transitions in the SLC23 elevator transporter UraA