PXD054381

PXD054381 is an original dataset announced via ProteomeXchange.

Title	Dysfunctional mitochondria trap proteins in the intermembrane space - Data set 5: MRP17 - AP-MS
Description	Mitochondria consist of hundreds of proteins which are initially synthesized in the cytosol as ‘precursors’ and imported into the organelle. The accumulation of precursors in the cytosol due to mitochondrial dysfunction compromises cellular proteostasis and is a hallmark of diseases. How cells counteract the accumulation of potentially toxic precursors in the cytosol is poorly understood. Using a proximity labeling-based assay to globally monitor the intramitochondrial location of proteins, we show, that upon mitochondrial dysfunction many mitochondrial matrix proteins are sequestered in the intermembrane space (IMS). This Mitochondrial Triage of Precursor proteins (MitoTraP) was not simply the result of a general translocation block at the level of the inner membrane. Rather, MitoTraP specifically directs a defined subgroup of matrix proteins into the IMS, most of which being constituents of the mitochondrial ribosome. Using the mitoribosomal protein Mrp17 (bS6m) as a model, we found that the targeting into the IMS prevents the mistargeting to the nucleolus and the interference with ribosome assembly. Thus, MitoTraP represents a novel, so far unknown mechanism of the eukaryotic quality control system that protects the cellular proteome again toxic effects of non-imported mitochondrial precursor proteins.
HostingRepository	PRIDE
AnnounceDate	2025-05-21
AnnouncementXML	Submission_2025-05-21_08:31:50.922.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Markus Räschle
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702; scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-30 03:20:02	ID requested
⏵ 1	2025-05-21 08:31:51	announced

Dataset with its publication pending

submitter keyword: yeast mitochondrial import

Markus Räschle
contact affiliation	Molecular Genetics, RPTU Kaiserslautern, Germany
contact email	raeschle@rhrk.uni-kl.de
lab head
Markus Räschle
contact affiliation	Technical University Kaiserslautern
contact email	raeschle@rhrk.uni-kl.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/05/PXD054381

PRIDE project URI

• PRIDE
  1. PXD054381
     1. Label: PRIDE project
     2. Name: Dysfunctional mitochondria trap proteins in the intermembrane space - Data set 5: MRP17 - AP-MS