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PXD054371

PXD054371 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleComparative proteomic landscapes provide insights into mammalian preimplantation development and failure
DescriptionUnderstanding mammalian preimplantation development, particularly in humans, at the proteomic level remains limited. Here, we applied our comprehensive solution of ultrasensitive proteomic technology to measure the proteomic profiles of oocytes and early embryos and identified nearly 8,000 proteins in humans and over 6,300 proteins in mice. We observed distinct proteomic dynamics before and around zygotic genome activation (ZGA) between the two species. Integrative analysis with translatomic data revealed extensive divergence between translation activation and protein accumulation. Multi-omic analysis indicated that ZGA transcripts often contribute to protein accumulation in blastocysts. Using mouse embryos, we identified several transcriptional regulators critical for early development, thereby linking ZGA to the first lineage specification. Furthermore, single-embryo proteomics of poor-quality embryos from over 100 patient couples provided insights into preimplantation development failure. Our study may contribute to reshaping the framework of mammalian preimplantation development and opening avenues for addressing human infertility.
HostingRepositoryPRIDE
AnnounceDate2025-02-05
AnnouncementXMLSubmission_2025-02-05_00:30:42.629.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChen Li
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListNo PTMs are included in the dataset
InstrumenttimsTOF Pro
Dataset History
RevisionDatetimeStatusChangeLog Entry
02024-07-30 00:58:59ID requested
12025-02-05 00:30:43announced
Publication List
Zhu W, Meng J, Li Y, Gu L, Liu W, Li Z, Shen Y, Shen X, Wang Z, Wu Y, Wang G, Zhang J, Zhang H, Yang H, Dong X, Wang H, Huang X, Sun Y, Li C, Mu L, Liu Z, Comparative proteomic landscapes elucidate human preimplantation development and failure. Cell, 188(3):814-831.e21(2025) [pubmed]
10.1016/j.cell.2024.12.028;
Keyword List
submitter keyword: Protein turnover, Mouse preimplantation development, Multi-omics analysis, Zygotic genome activation (ZGA),Ultrasensitive proteomics, Cross species analysis
Contact List
Chen Li
contact affiliationCenter for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University Schoole of Medicine
contact emailcli@shsmu.edu.cn
lab head
Chen Li
contact affiliationCenter for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine
contact emailcli@shsmu.edu.cn
dataset submitter
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Dataset FTP location
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