PXD054371

PXD054371 is an original dataset announced via ProteomeXchange.

Title	Comparative proteomic landscapes provide insights into mammalian preimplantation development and failure
Description	Understanding mammalian preimplantation development, particularly in humans, at the proteomic level remains limited. Here, we applied our comprehensive solution of ultrasensitive proteomic technology to measure the proteomic profiles of oocytes and early embryos and identified nearly 8,000 proteins in humans and over 6,300 proteins in mice. We observed distinct proteomic dynamics before and around zygotic genome activation (ZGA) between the two species. Integrative analysis with translatomic data revealed extensive divergence between translation activation and protein accumulation. Multi-omic analysis indicated that ZGA transcripts often contribute to protein accumulation in blastocysts. Using mouse embryos, we identified several transcriptional regulators critical for early development, thereby linking ZGA to the first lineage specification. Furthermore, single-embryo proteomics of poor-quality embryos from over 100 patient couples provided insights into preimplantation development failure. Our study may contribute to reshaping the framework of mammalian preimplantation development and opening avenues for addressing human infertility.
HostingRepository	PRIDE
AnnounceDate	2025-02-05
AnnouncementXML	Submission_2025-02-05_00:30:42.629.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Chen Li
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	timsTOF Pro

Revision	Datetime	Status	ChangeLog Entry
0	2024-07-30 00:58:59	ID requested
⏵ 1	2025-02-05 00:30:43	announced

Zhu W, Meng J, Li Y, Gu L, Liu W, Li Z, Shen Y, Shen X, Wang Z, Wu Y, Wang G, Zhang J, Zhang H, Yang H, Dong X, Wang H, Huang X, Sun Y, Li C, Mu L, Liu Z, Comparative proteomic landscapes elucidate human preimplantation development and failure. Cell, 188(3):814-831.e21(2025) [pubmed]

10.1016/j.cell.2024.12.028;

submitter keyword: Protein turnover, Mouse preimplantation development, Multi-omics analysis, Zygotic genome activation (ZGA),Ultrasensitive proteomics, Cross species analysis

Chen Li
contact affiliation	Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University Schoole of Medicine
contact email	cli@shsmu.edu.cn
lab head
Chen Li
contact affiliation	Center for Single-Cell Omics, School of Public Health, Shanghai Jiao Tong University School of Medicine
contact email	cli@shsmu.edu.cn
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2025/02/PXD054371

PRIDE project URI

• PRIDE
  1. PXD054371
     1. Label: PRIDE project
     2. Name: Comparative proteomic landscapes provide insights into mammalian preimplantation development and failure